Transoral intratracheal inoculation method for use with neonatal rats

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:54Z
Fecha de publicación2001-01-01
ResumenBackground and Purpose: Studying the effects of toxic and infective compounds on the respiratory system requires a reliable method for delivering inoculum into the distal region of the lung. Although transoral intratracheal inoculation methods have been well documented for adult rats, to the authors' knowledge, a reliable method has not been validated for neonatal rats. The purpose of the study reported here was to develop a simple method for transoral inoculation in rat neonates, Methods: Seven-day-old Fischer 344 rats were anesthetized with halothane, and a spinal needle was inserted in the tracheal lumen, by use of illumination and a modified otoscope, Meconium was injected into the lungs as a marker, and the neonates were kept under close observation After euthanasia at 24 h, lungs were removed and fixed in formalin, and the microscopic distribution of the inoculum was assessed in the left, right cranial, middle, median, and caudal lung lobes. Results: Microscopic examination of lungs indicated that intratracheal inoculation was achieved in 100\% of neonatal, lungs and the inoculum was consistently distributed in the alveoli of all pulmonary lobes, Important complications or mortality were not observed in the neonates, Conclusions: Intratracheal inoculation of neonatal rats is possible by use of a modified otoscope for transoral illumination. This technique is simple and reproducible and ensures, without complications, widespread distribution of inoculum in the lungs of neonatal rats.es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4721
Idiomaenes_ES
EditorialAMER ASSOC LABORATORY ANIMAL SCIENCEes_ES
RelaciónComparative Medicinees_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteComparative Medicine
TítuloTransoral intratracheal inoculation method for use with neonatal ratses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorMartínez-Burnes, J
AutorLópez, A
AutorLemke, K
AutorDobbin, G
AutorMartínez-Burnes, Jes_ES
AutorLópez, Aes_ES
AutorLemke, Kes_ES
AutorDobbin, Ges_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número2es_ES
Rango de páginas134-137es_ES
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen51es_ES

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