Accuracy of the Phenotypic 1G Test to Detect Mycobacterium tuberculosis and Drug Resistance From Sputa in the US-Mexico Border

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:40Z
Fecha de publicación2026-01-01
ResumenBackground.With >10 million new tuberculosis (TB) cases per year, a limitation to TB control is the lack of simple and accurate tests for diagnosis and drug susceptibility testing (DST) in endemic regions. We evaluated the accuracy of the first-generation, low-complexity phenotypic TB test (1G test), designed for simultaneous Mycobacterium tuberculosis (Mtb) detection and resistance to isoniazid, rifampicin, and moxifloxacin, suitable for resource-limited settings. Methods.A cross-sectional study was conducted with sputa from 426 possible pulmonary TB cases from 2 small Mexican cities bordering Texas. The 1G test was compared against phenotypic TB detection tests in the region, specifically acid-fast bacilli smear microscopy and Mycobacteria Growth Indicator Tube (MGIT) culture, as well as MGIT-DST for resistance to isoniazid, rifampicin, and moxifloxacin. Results.The 1G test demonstrated >= 98\% sensitivity for Mtb detection; 100\% sensitivity and 91\% (rifampicin), 94\% (isoniazid), and 97\% (moxifloxacin) specificity for DST; and less contamination than the MGIT (3.5\% vs 8.1\%, P < .05). The 1G test time to detection of Mtb and simultaneous DST was 17 days, while the MGIT-DST required 2 steps: 7 days for Mtb detection plus 14 more days for DST (21 days total). Our study site drug-resistant TB prevalence was 14\% when testing all consecutively enrolled participants vs 6\% by passive reporting. Conclusions. The 1G test is a low-complexity phenotypic TB diagnostic method that is a practical replacement to current culture-based tests. Future studies are warranted to evaluate implementation of the 1G test in decentralized clinics that lack molecular tools, resources, and expertise.es_ES
Doihttps://doi.org/10.1093/infdis/jiaf638es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5487
Idiomaenes_ES
EditorialOXFORD UNIV PRESS INCes_ES
RelaciónJournal of Infectious Diseaseses_ES
URL relacionadohttps://doi.org/10.1093/infdis/jiaf638es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteJournal of Infectious Diseases
Palabra claveculturees_ES
Palabra clavediagnosises_ES
Palabra claveDSTes_ES
Palabra clavephenotypices_ES
Palabra clavetuberculosises_ES
TítuloAccuracy of the Phenotypic 1G Test to Detect Mycobacterium tuberculosis and Drug Resistance From Sputa in the US-Mexico Borderes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAguirre, Mia
AutorAyala, Doris
AutorGarcia, Juan Ignacio
AutorMartinez-Lopez, Yoscelina E.
AutorHicks, Amberlee D.
AutorChacon, Nadine
AutorGay-Cobb, Ashley
AutorSchami, Alyssa
AutorZavala-Perez, Selena
AutorDominguez-Trejo, Ilse A.
AutorCruz-Gonzalez, America M.
AutorLoera-Salazar, Raul
AutorRodriguez-Herrera, Javier E.
AutorGarcia-Oropesa, Esperanza M.
AutorLee, Miryoung
AutorRendon, Adrian
AutorWang, Shu-Hua
AutorYotebieng, Marcel
AutorEvans, Carlton A.
AutorTorrelles, Jordi B.
AutorRestrepo, I, Blanca
AutorAguirre, Miaes_ES
AutorAyala, Dorises_ES
AutorGarcia, Juan Ignacioes_ES
AutorMartinez-Lopez, Yoscelina E.es_ES
AutorHicks, Amberlee D.es_ES
AutorChacon, Nadinees_ES
AutorGay-Cobb, Ashleyes_ES
AutorSchami, Alyssaes_ES
AutorZavala-Perez, Selenaes_ES
AutorDominguez-Trejo, Ilse A.es_ES
AutorCruz-Gonzalez, America M.es_ES
AutorLoera-Salazar, Raules_ES
AutorRodriguez-Herrera, Javier E.es_ES
AutorGarcia-Oropesa, Esperanza M.es_ES
AutorLee, Miryounges_ES
AutorRendon, Adrianes_ES
AutorWang, Shu-Huaes_ES
AutorYotebieng, Marceles_ES
AutorEvans, Carlton A.es_ES
AutorTorrelles, Jordi B.es_ES
AutorRestrepo, I, Blancaes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número4es_ES
Rango de páginase973-e980es_ES
URL relacionadahttps://doi.org/10.1093/infdis/jiaf638
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen233es_ES

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