A pyrosequencing method for molecular monitoring of regions in the inhA, ahpC and rpoB genes of Mycobacterium tuberculosis

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:02Z
Fecha de publicación2010-01-01
ResumenIn this study, a pyrosequencing method for monitoring two genes related to isoniazid (INH)-resistance and a region of the rpoB gene linked to rifampin (RMP)-resistance in Mycobacterium tuberculosis was developed and evaluated. Specifically, a 20-base pair (bp) region of inhA (from -24 to -4), a 35-bp region of ahpC (from -39 to -4), and a 57-bp region of rpoB (from codon 515 to 533) were analysed by pyrosequencing. For the development of the method, selected non-consecutive clinical isolates of M. tuberculosis were analysed, including: 25 isolates susceptible to both INH and RMP, 18 RMP-monoresistant isolates, 17 INH-monoresistant isolates, and 15 multi-drug-resistant strains. Our pyrosequencing methodology was further evaluated using 96 M. tuberculosis isolates. Mutations in ahpC were found to be associated with INH resistance (p <0.05). By setting any mutation in ahpC as a marker of resistance, the specificity and the positive predictive value (PPV) were 100\%. Similarly, any mutation in the rpoB gene was associated with a RMP resistance phenotype (p <0.01). Using any mutation in rpoB as a marker of RMP resistance, the sensitivity of this assay was 73\% and the specificity and PPV were 100\%. The use of this pyrosequencing method to analyse the ahpC and rpoB genes allowed us to detect INH-and/or RMP-resistant isolates. Furthermore, this method represents an opportunity to expedite the description of novel mutations related to drug resistance.es_ES
Doihttps://doi.org/10.1111/j.1469-0691.2009.02932.xes_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4873
Idiomaenes_ES
EditorialWILEY-BLACKWELLes_ES
RelaciónClinical Microbiology and Infectiones_ES
URL relacionadohttps://doi.org/10.1111/j.1469-0691.2009.02932.xes_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteClinical Microbiology and Infection
Palabra claveAhpCes_ES
Palabra claveinhAes_ES
Palabra claveMycobacterium tuberculosises_ES
Palabra clavepyrosequencinges_ES
Palabra claverpoBes_ES
TítuloA pyrosequencing method for molecular monitoring of regions in the inhA, ahpC and rpoB genes of Mycobacterium tuberculosises_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorGarza-Gonzalez, E.
AutorGonzalez, G. M.
AutorRenteria, A.
AutorCruz-Pulido, W.
AutorRivera, G.
AutorBocanegra-Garcia, V.
AutorGarza-Gonzalez, E.es_ES
AutorGonzalez, G. M.es_ES
AutorRenteria, A.es_ES
AutorCruz-Pulido, W.es_ES
AutorRivera, G.es_ES
AutorBocanegra-Garcia, V.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número6es_ES
Rango de páginas607-612es_ES
URL relacionadahttps://doi.org/10.1111/j.1469-0691.2009.02932.x
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen16es_ES

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