Unravelling the physicochemical and antimicrobial mechanisms of human serum albumin/tannic acid coatings for medical-grade polycaprolactone scaffolds

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:38Z
Fecha de publicación2024-01-01
ResumenBiofilm-related biomaterial infections are notoriously challenging to treat and can lead to chronic infection and persisting inflammation. To date, a large body of research can be reviewed for coatings which potentially prevent bacterial infection while promoting implant integration. Yet only a very small number has been translated from bench to bedside. This study provides an in-depth analysis of the stability, antibacterial mechanism, and biocompatibility of medical grade polycaprolactone (mPCL), coated with human serum albumin (HSA), the most abundant protein in blood plasma, and tannic acid (TA), a natural polyphenol with antibacterial properties. Molecular docking studies demonstrated that HSA and TA interact mainly through hydrogen-bonding, ionic and hydrophobic interactions, leading to smooth and regular assemblies. In vitro bacteria adhesion testing showed that coated scaffolds maintained their antimicrobial properties over 3 days by significantly reducing S. aureus colonization and biofilm formation. Notably, amplitude modulation-frequency modulation (AMFM) based viscoelasticity mapping and transmission electron microscopy (TEM) data suggested that HSA/TA-coatings cause morphological and mechanical changes on the outer cell membrane of S. aureus leading to membrane disruption and cell death while proving non-toxic to human primary cells. These results support this antibiotic-free approach as an effective and biocompatible strategy to prevent biofilm-related biomaterial infections.es_ES
Doihttps://doi.org/10.1016/j.bioactmat.2024.08.023es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5433
Idiomaenes_ES
EditorialKEAI PUBLISHING LTDes_ES
RelaciónBioactive Materialses_ES
URL relacionadohttps://doi.org/10.1016/j.bioactmat.2024.08.023es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteBioactive Materials
Palabra claveBacterial infectiones_ES
Palabra claveBiofilmes_ES
Palabra claveHuman serum albumines_ES
Palabra claveTannic acides_ES
Palabra clavePolycaprolactonees_ES
Palabra clave3D printinges_ES
Palabra claveScaffoldes_ES
TítuloUnravelling the physicochemical and antimicrobial mechanisms of human serum albumin/tannic acid coatings for medical-grade polycaprolactone scaffoldses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorCometta, Silvia
AutorDonose, Bogdan C.
AutorJuarez-Saldivar, Alfredo
AutorRavichandran, Akhilandeshwari
AutorXu, Yanan
AutorBock, Nathalie
AutorDargaville, Tim R.
AutorRakic, Aleksandar D.
AutorHutmacher, Dietmar W.
AutorCometta, Silviaes_ES
AutorDonose, Bogdan C.es_ES
AutorJuarez-Saldivar, Alfredoes_ES
AutorRavichandran, Akhilandeshwaries_ES
AutorXu, Yananes_ES
AutorBock, Nathaliees_ES
AutorDargaville, Tim R.es_ES
AutorRakic, Aleksandar D.es_ES
AutorHutmacher, Dietmar W.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Rango de páginas68-84es_ES
URL relacionadahttps://doi.org/10.1016/j.bioactmat.2024.08.023
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen42es_ES

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