Elisidepsin Interacts Directly with Glycosylceramides in the Plasma Membrane of Tumor Cells to Induce Necrotic Cell Death.

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxico [143]es_ES
Fecha de ingreso2026-09-09T01:06:07Z
Fecha de publicación2015-01-01
ResumenPlasma membrane integrity is essential for cell life. Any major break on it immediately induces the death of the affected cell. Different molecules were described as disrupting this cell structure and thus showing antitumor activity. We have previously defined that elisidepsin (Irvalec®, PM02734) inserts and self-organizes in the plasma membrane of tumor cells, inducing a rapid loss of membrane integrity, cell permeabilization and necrotic death. Here we show that, in sensitive HCT-116 colorectal cells, all these effects are consequence of the interaction of elisidepsin with glycosylceramides in the cell membrane. Of note, an elisidepsin-resistant subline (HCT-116-Irv) presented reduced levels of glycosylceramides and no accumulation of elisidepsin in the plasma membrane. Consequently, drug treatment did not induce the characteristic necrotic cell death. Furthermore, GM95, a mutant derivative from B16 mouse melanoma cells lacking ceramide glucosyltransferase (UGCG) activity and thus the synthesis of glycosylceramides, was also resistant to elisidepsin. Over-expression of UGCG gene in these deficient cells restored glycosylceramides synthesis, rendering them sensitive to elisidepsin, at a similar level than parental B16 cells. These results indicate that glycosylceramides act as membrane targets of elisidepsin, facilitating its insertion in the plasma membrane and the subsequent membrane permeabilization that leads to drug-induced cell death. They also indicate that cell membrane lipids are a plausible target for antineoplastic therapy.es_ES
Doi10.1371/journal.pone.0140782es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/1540
IdiomaEspañol [65]es_ES
RelaciónJosé Manuel Molina-Guijarro, Carolina García, Álvaro Macías, Luis Francisco García-Fernández, Cristina Moreno, Fernando Reyes, et al.. (2015). Elisidepsin Interacts Directly with Glycosylceramides in the Plasma Membrane of Tumor Cells to Induce Necrotic Cell Death.. PLoS ONE. https://doi.org/10.1371/journal.pone.0140782es_ES
URL relacionadohttp://europepmc.org/articles/PMC4608773?pdf=renderes_ES
DerechosAcceso Abiertoes_ES
LicenciaBY NC NDes_ES
FuentePLoS ONE, () vol.? (2015)
ClasificaciónCIENCIAS SOCIALES [5]:CIENCIAS ECONÓMICAS [53]:ORGANIZACIÓN Y DIRECCIÓN DE EMPRESAS [5311]es_ES
TítuloElisidepsin Interacts Directly with Glycosylceramides in the Plasma Membrane of Tumor Cells to Induce Necrotic Cell Death.es_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
ÁreaCIENCIAS SOCIALES [5]
ÁreaCIENCIAS SOCIALES [5]es_ES
AutorJOSÉ MANUEL MOLINA-GUIJARRO
AutorCAROLINA GARCÍA
AutorÁLVARO MACÍAS
AutorLUIS FRANCISCO GARCÍA-FERNÁNDEZ
AutorCRISTINA MORENO
AutorFERNANDO REYES
AutorJUAN FERNANDO MARTÍNEZ-LEAL
AutorROGELIO FERNÁNDEZ
AutorVALENTÍN MARTÍNEZ
AutorCARMEN VALENZUELA
AutorM PILAR LILLO
AutorCARLOS M GALMARINI
AutorJOSÉ MANUEL MOLINA-GUIJARROes_ES
AutorCAROLINA GARCÍAes_ES
AutorÁLVARO MACÍASes_ES
AutorLUIS FRANCISCO GARCÍA-FERNÁNDEZes_ES
AutorCRISTINA MORENOes_ES
AutorFERNANDO REYESes_ES
AutorJUAN FERNANDO MARTÍNEZ-LEALes_ES
AutorROGELIO FERNÁNDEZes_ES
AutorVALENTÍN MARTÍNEZes_ES
AutorCARMEN VALENZUELAes_ES
AutorM PILAR LILLOes_ES
AutorCARLOS M GALMARINIes_ES
DisciplinaCIENCIAS ECONÓMICAS [53]es_ES
EdiciónPrimeraes_ES
SubdisciplinaORGANIZACIÓN Y DIRECCIÓN DE EMPRESAS [5311]es_ES
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES

Files

Collections

Documentación Cientifica