Bisphenol A concentration-dependently increases human granulosa-lutein cell matrix metalloproteinase-9 (MMP-9) enzyme output

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:54Z
Fecha de publicación2008-01-01
ResumenBisphenol A (BPA) is an estrogenic contaminant that has been quantified at higher levels in the follicular fluid of women with polycystic ovarian syndrome (PCOS) compared to healthy fertile controls. However, the effect of BPA on granulosa cell function is unknown. Therefore, the objective of the present study was to quantify the effect of BPA on granulosa cell progesterone (P4) output and matrix metalloproteinase (MMP)-2, and -9 output and activity. Granulosa-lutein cells (GLCs) were collected from women undergoing oocyte retrieval in an academic in vitro fertilization (IVF) program. Granulosa-lutein cells were treated with increasing log concentrations of BPA (1-10,000 ng/ml) or 17 beta-estradiol (E-2, 272 pg/ml or 1.0nM) and treatment effects on MMP-2 and -9 activity and output, cell viability and cell proliferation were measured by commercial gelatin zymography, MMP-ELISA, MTS and BrdU incorporation assays, respectively. Granulosa-lutein cells in culture secrete MMP-2 and MMP-9. Bisphenol A treatment concentration-dependently increased MMP-9 output by GLCs with a maximal effect observed at 1000 ng/ml. Cell viability/proliferation was unaffected by BPA treatment at concentrations <= 100 ng/ml; however, higher concentrations of BPA were cytotoxic. Progesterone Output by the GLCs was unaffected by increasing BPA concentrations in the media. In conclusion, GLCs in culture secrete MMP-2 and MMP-9. At lower concentrations, compatible with human exposure levels (100-1000 ng/ml), BPA stimulates GLC MMP-9 output; however, higher concentrations are cytotoxic. Our findings suggest that BPA treatment can modulate ovarian extracellular matrix stability. (C) 2008 Elsevier Inc. All rights reserved.es_ES
Doihttps://doi.org/10.1016/j.reprotox.2008.05.059es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4733
Idiomaenes_ES
EditorialPERGAMON-ELSEVIER SCIENCE LTDes_ES
RelaciónReproductive Toxicologyes_ES
URL relacionadohttps://doi.org/10.1016/j.reprotox.2008.05.059es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteReproductive Toxicology
Palabra clavebisphenol Aes_ES
Palabra clavegranulosa cellses_ES
Palabra clavematrix metalloproteinaseses_ES
Palabra claveMMP-9es_ES
Palabra clavefolliculogenesises_ES
TítuloBisphenol A concentration-dependently increases human granulosa-lutein cell matrix metalloproteinase-9 (MMP-9) enzyme outputes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorDominguez, Miguel A.
AutorPetre, Maria A.
AutorNeal, Michael S.
AutorFoster, Warren G.
AutorDominguez, Miguel A.es_ES
AutorPetre, Maria A.es_ES
AutorNeal, Michael S.es_ES
AutorFoster, Warren G.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número4es_ES
Rango de páginas420-425es_ES
URL relacionadahttps://doi.org/10.1016/j.reprotox.2008.05.059
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen25es_ES

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