A Novel Tannase from the Xerophilic Fungus Aspergillus niger GH1

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:44Z
Fecha de publicación2009-01-01
ResumenAspergillus niger GH1 previously isolated and identified by our group as a wild tannase producer was grown under solid-state (SSC) and submerged culture (SmC) conditions to select the enzyme production system. For tannase purification, extracellular tannase was produced under SSC using polyurethane foam as the inert support. Tannase was purified to apparent homogeneity by ultrafiltration, anion-exchange chromatography, and gel filtration that led to a purified enzyme with a specific activity of 238.14 IU/mg protein with a final yield of 0.3\% and a purification fold of 46. Three bands were found on the SDS-PAG with molecular masses of 50, 75, and 100 kDa. PI of 3.5 and 7.1\% N-glycosylation were noted. Temperature and pH optima were 60 degrees C and 6.0 {[}methyl 3,4,5-trihydroxybenzoate (MTB) as substrate], respectively. Tannase was found with a Km value of 0.41 x 10(-4) M and the value of V-max was 11.03 mu moL/min at 60 degrees C for MTB. Effects of several metal salts, solvents, surfactants, and typical enzyme inhibitors on tannase activity were evaluated to establish the novelty of the enzyme. Finally, the tannase from A. niger GH1 was significantly inhibited by PMSF (phenylmethylsulfonyl fluoride), and therefore, it is possible to consider the presence of a serine or cysteine residue in the catalytic site.es_ES
Doihttps://doi.org/10.4014/jmb.0811.615es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4577
Idiomaenes_ES
EditorialKOREAN SOC MICROBIOLOGY \& BIOTECHNOLOGYes_ES
RelaciónJournal of Microbiology and Biotechnologyes_ES
URL relacionadohttps://doi.org/10.4014/jmb.0811.615es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteJournal of Microbiology and Biotechnology
Palabra claveAspergillus niger GH1 tannasees_ES
Palabra clavepropertieses_ES
Palabra claveproductiones_ES
Palabra clavepurificationes_ES
Palabra clavesolid-state culturees_ES
TítuloA Novel Tannase from the Xerophilic Fungus Aspergillus niger GH1es_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorMarco, Mata-Gomez
AutorRodriguez, Luis V.
AutorRamos, Erika L.
AutorRenovato, Jacqueline
AutorCruz-Hernandez, Mario A.
AutorRodriguez, Raul
AutorContreras, Juan
AutorAguilar, Cristobal N.
AutorMarco, Mata-Gomezes_ES
AutorRodriguez, Luis V.es_ES
AutorRamos, Erika L.es_ES
AutorRenovato, Jacquelinees_ES
AutorCruz-Hernandez, Mario A.es_ES
AutorRodriguez, Raules_ES
AutorContreras, Juanes_ES
AutorAguilar, Cristobal N.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número9es_ES
Rango de páginas987-996es_ES
URL relacionadahttps://doi.org/10.4014/jmb.0811.615
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen19es_ES

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