Silencing ACE1 Gene with dsRNA of Different Lengths Impairs Larval Development in Leptinotarsa decemlineata

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:32Z
Fecha de publicación2024-01-01
ResumenIn the search for effective strategies to control the Colorado Potato Beetle, RNA interference technology has emerged as a promising method due to its capacity to suppress genes selectively. Factors such as the target gene and double-stranded RNA (dsRNA) length are critical for optimizing gene silencing efficiency. In this study, we designed and synthesized in vitro dsRNAs of varying lengths targeting the ACE1 gene, which encodes the AChE1 isoform of acetylcholinesterase in the beetle. All tested dsRNA lengths (222 bp, 543 bp, 670 bp, and 870 bp) promoted transcript reduction. The 670 bp dsRNA was the most effective, reducing transcript levels by approximately 40\% by day seven, followed by the 543 bp dsRNA. No significant differences were observed between the 222 bp and 870 bp dsRNAs. Furthermore, all of the dsRNA lengths resulted in reduced weight gain and increased mortality in larvae, with the 670 bp dsRNA showing the highest mortality rate, leaving only 63\% larval survival, a trend that persisted through day nine. These findings emphasize that dsRNA length is a key factor in the silencing response, underscoring the importance of selecting the optimal length while considering the gene's target, stability, and delivery methods. This study contributes to establishing design criteria for dsRNA, aiding in the development of more effective and sustainable pest management strategies.es_ES
Doihttps://doi.org/10.3390/insects15121000es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5351
Idiomaenes_ES
EditorialMDPIes_ES
RelaciónInsectses_ES
URL relacionadohttps://doi.org/10.3390/insects15121000es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteInsects
Palabra clave<italic>Leptinotarsa decemlineata</italic>es_ES
Palabra claveacetylcholinesterasees_ES
Palabra clave<italic>ACE1</italic> genees_ES
Palabra claveRNA interferencees_ES
Palabra clavedsRNA lengthes_ES
Palabra clavetranscript levelses_ES
Palabra clavemortalityes_ES
TítuloSilencing ACE1 Gene with dsRNA of Different Lengths Impairs Larval Development in Leptinotarsa decemlineataes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorJulian-Chavez, Brenda
AutorSiqueiros-Cendon, Tania S.
AutorTorres-Castillo, Jorge Ariel
AutorSinagawa-Garcia, Sugey Ramona
AutorAbraham-Juarez, Maria Jazmin
AutorGonzalez-Barriga, Carmen Daniela
AutorRascon-Cruz, Quintin
AutorSianez-Estrada, Luis Ignacio
AutorArevalo-Gallegos, Sigifredo
AutorEspinoza-Sanchez, Edward Alexander
AutorJulian-Chavez, Brendaes_ES
AutorSiqueiros-Cendon, Tania S.es_ES
AutorTorres-Castillo, Jorge Arieles_ES
AutorSinagawa-Garcia, Sugey Ramonaes_ES
AutorAbraham-Juarez, Maria Jazmines_ES
AutorGonzalez-Barriga, Carmen Danielaes_ES
AutorRascon-Cruz, Quintines_ES
AutorSianez-Estrada, Luis Ignacioes_ES
AutorArevalo-Gallegos, Sigifredoes_ES
AutorEspinoza-Sanchez, Edward Alexanderes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número12es_ES
URL relacionadahttps://doi.org/10.3390/insects15121000
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen15es_ES

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