Differential Expression of the Tick Protective Antigen Subolesin in Anaplasma marginale‐ and A. phagocytophilum‐infected Host Cells

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:30:37Z
Fecha de publicación2008-12-01
ResumenSubolesin was recently shown in vaccine and RNA interference (RNAi) studies to protect against tick infestations and to affect tick feeding, reproduction, and development as well as infection of host cells by Anaplasma marginale and A. phagocytophilum. Recent experiments provided evidence that infection of both tick and vertebrate host cells with these two pathogens modified gene expression. We therefore hypothesized that infection of host cells with A. marginale and A. phagocytophilum affects expression of subolesin. Subolesin mRNA levels were determined by real-time reverse transcriptase (RT)-PCR in uninfected and A. marginale-infected Dermacentor variabilis guts and salivary glands and IDE8-cultured tick cells and in uninfected and A. phagocytophilum-infected Ixodes scapularis nymphs, ISE6-cultured tick cells, and the human cell line HL-60. In addition, the effect of subolesin on Anaplasma spp. infection/multiplication was characterized by RNAi in tick tissues and/or cultured tick and human cells. These experiments presented evidence of differential expression of subolesin in A. marginale- and A. phagocytophilum-infected cells. Subolesin was differentially expressed in A. marginale-infected ticks in a tissue-specific manner in which mRNA levels increased in response to A. marginale infection in tick salivary gland cells but not in the gut cells. Subolesin knockdown by RNAi reduced Anaplasma infection/multiplication only in cells in which infection increased subolesin expression, i.e., in A. marginale-infected D. variabilis salivary glands and IDE8 cells. The results reported herein further support the role of subolesin in Anaplasma-host interactions and suggest a putative role of subolesin in vaccines for the control of pathogen infection/multiplication in ticks.es_ES
Doihttps://doi.org/10.1196/annals.1428.056es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2594
Idiomaenes_ES
EditorialWileyes_ES
RelaciónAnnals of the New York Academy of Scienceses_ES
URL relacionadohttps://doi.org/10.1196/annals.1428.056es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteAnnals of the New York Academy of Sciences
Palabra claveAnaplasma phagocytophilumes_ES
Palabra claveBiologyes_ES
Palabra claveTickes_ES
Palabra claveIxodes scapularises_ES
Palabra claveDermacentor variabilises_ES
Palabra claveVirologyes_ES
Palabra claveAnaplasmaes_ES
Palabra claveGene knockdownes_ES
Palabra claveAntigenes_ES
Palabra claveRNA interferencees_ES
Palabra claveAnaplasmataceaees_ES
Palabra claveMicrobiologyes_ES
ClasificaciónVector-borne infectious diseaseses_ES
TítuloDifferential Expression of the Tick Protective Antigen Subolesin in Anaplasma marginale‐ and A. phagocytophilum‐infected Host Cellses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorFuente, José de la
AutorBlouin, Edmour F.
AutorManzano-Román, Raúl
AutorNaranjo, Victoria
AutorAlmazán, Consuelo
AutorLastra, José Manuel Pérez de la
AutorŽivković, Zorica
AutorMassung, Robert F.
AutorJongejan, Frans
AutorKocan, Katherine M.
AutorFuente, José de laes_ES
AutorBlouin, Edmour F.es_ES
AutorManzano-Román, Raúles_ES
AutorNaranjo, Victoriaes_ES
AutorAlmazán, Consueloes_ES
AutorLastra, José Manuel Pérez de laes_ES
AutorŽivković, Zoricaes_ES
AutorMassung, Robert F.es_ES
AutorJongejan, Franses_ES
AutorKocan, Katherine M.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número1es_ES
Rango de páginas27-35es_ES
URL relacionadahttps://doi.org/10.1196/annals.1428.056
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen1149es_ES

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