Gene Silencing of Sarco/Endoplasmic Reticulum Ca2+-ATPase and NADPH-Cytochrome P450 Reductase as a Novel Approach to Leptinotarsa decemlineata Management

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:31Z
Fecha de publicación2025-01-01
ResumenThe Colorado Potato Beetle (CPB, Leptinotarsa decemlineata Say, Coleoptera: Chrysomelidae) remains a destructive agricultural pest worldwide that continually overcomes conventional control methods. In recent years, RNA interference (RNAi) has emerged as an alternative for its management; however, although promising results have been reported, its effectiveness has been influenced by several factors, including the length of double-stranded RNA (dsRNA), the delivery method, stability, and especially the selection of the target gene. In this study, we designed and synthesized 290 bp dsRNAs targeting the SERCA and CPR genes from L. decemlineata, which encode the Sarco/Endoplasmic Reticulum Ca2+-ATPase and NADPH-Cytochrome P450 Reductase, respectively. Both dsRNAs successfully reduced transcript levels in larvae, with dsSERCA achieving similar to 60\% knockdown by day 3 and dsCPR achieving similar to 50\% knockdown by day 7. Furthermore, both treatments affected the larval growth and survival rate. However, while the dsCPR-treated larvae showed a 59\% reduction in weight gain, the administration of dsSERCA had a strong phenotypic effect on the larvae, leading to decreased feeding, a 50.4\% reduction in weight gain, and ultimately, 100\% mortality. These results suggest that the SERCA and CPR genes could be promising targets for L. decemlineata control and emphasize the importance of appropriate target gene selection for RNAi silencing, as well as the need to explore and validate new genes for RNAi-mediated pest management.es_ES
Doihttps://doi.org/10.3390/agronomy15051151es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5331
Idiomaenes_ES
EditorialMDPIes_ES
RelaciónAgronomyes_ES
URL relacionadohttps://doi.org/10.3390/agronomy15051151es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteAgronomy
Palabra claveLeptinotarsa decemlineataes_ES
Palabra claveSERCA genees_ES
Palabra claveCPR genees_ES
Palabra claveRNA interferencees_ES
Palabra clavetranscript levelses_ES
Palabra clavemortalityes_ES
TítuloGene Silencing of Sarco/Endoplasmic Reticulum Ca2+-ATPase and NADPH-Cytochrome P450 Reductase as a Novel Approach to Leptinotarsa decemlineata Managementes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorMendoza-Alatorre, Maribel
AutorSiqueiros-Cendon, Tania Samanta
AutorTorres-Castillo, Jorge Ariel
AutorSinagawa-Garcia, Sugey Ramona
AutorIglesias-Figueroa, Blanca Flor
AutorAbraham-Juarez, Maria Jazmin
AutorGonzalez-Barriga, Carmen Daniela
AutorRascon-Cruz, Quintin
AutorSianez-Estrada, Luis Ignacio
AutorEspinoza-Sanchez, Edward Alexander
AutorMendoza-Alatorre, Maribeles_ES
AutorSiqueiros-Cendon, Tania Samantaes_ES
AutorTorres-Castillo, Jorge Arieles_ES
AutorSinagawa-Garcia, Sugey Ramonaes_ES
AutorIglesias-Figueroa, Blanca Flores_ES
AutorAbraham-Juarez, Maria Jazmines_ES
AutorGonzalez-Barriga, Carmen Danielaes_ES
AutorRascon-Cruz, Quintines_ES
AutorSianez-Estrada, Luis Ignacioes_ES
AutorEspinoza-Sanchez, Edward Alexanderes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número5es_ES
URL relacionadahttps://doi.org/10.3390/agronomy15051151
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen15es_ES

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