Mannheimia haemolytica A2 secretes different proteases into the culture medium and in outer membrane vesicles

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:03Z
Fecha de publicación2017-01-01
ResumenRespiratory diseases in ruminants have a significantly negative impact on the worldwide economy. The bacterium Mannheimia haemolytica is involved in pneumonic infections in bovine and ovine. In gram-negative bacteria, six secretion systems related to the colonization process and host tissue damage have been reported. In addition, in the last two decades, the production of outer membrane vesicles has been studied as a different bacterial strategy to release virulence factors, such as exotoxins, lipopolysaccharides, and proteases. However, in M. haemolytica serotype A2, protease secretion and release in vesicles have not been reported as virulence mechanisms. The aim of this work was to identify proteases released into the culture supernatant and in vesicles of M. haemolytica A2. Our results showed evident differences in the molecular mass and activity of proteases present in culture supernatants and outer membrane vesicles based on zymography assays. The biochemical characterization of M. haemolytica proteases revealed that the main types were cysteine and metalloproteases. A specific metalloprotease of 100 kDa was active in the culture supernatants, but it was not active and was found in low quantities in vesicles. Proteases could be an important virulence factor during the infectious pneumonic process led by M. haemolytica.es_ES
Doihttps://doi.org/10.1016/j.micpath.2017.10.027es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4892
Idiomaenes_ES
EditorialACADEMIC PRESS LTD- ELSEVIER SCIENCE LTDes_ES
RelaciónMicrobial Pathogenesises_ES
URL relacionadohttps://doi.org/10.1016/j.micpath.2017.10.027es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteMicrobial Pathogenesis
Palabra claveMannheimia haemolyticaes_ES
Palabra claveOuter membrane vesicleses_ES
Palabra claveProteaseses_ES
Palabra claveZymogramses_ES
TítuloMannheimia haemolytica A2 secretes different proteases into the culture medium and in outer membrane vesicleses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorRamirez Rico, Gerardo
AutorMartinez-Castillo, Moises
AutorGonzalez-Ruiz, Cynthia
AutorLuna-Castro, Sarahi
Autorde la Garza, Mireya
AutorRamirez Rico, Gerardoes_ES
AutorMartinez-Castillo, Moiseses_ES
AutorGonzalez-Ruiz, Cynthiaes_ES
AutorLuna-Castro, Sarahies_ES
Autorde la Garza, Mireyaes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Rango de páginas276-281es_ES
URL relacionadahttps://doi.org/10.1016/j.micpath.2017.10.027
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen113es_ES

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