Multiplex PCR for detection of bacterial uropathogens in nosocomial samples from northeastern Mexico: one health-relevant urinary tract pathogens

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:30:08Z
Fecha de publicación2026-01-01
ResumenUrinary tract infections (UTIs) are among the most common bacterial infections worldwide, accounting for approximately 150 million cases annually and an estimated 3.8 million cases each year in Mexico. The principal uropathogens are Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, all of which are opportunistic zoonotic bacteria associated with humans, companion animals, livestock, and wildlife. Within the One Health framework, these pathogens and their antimicrobial resistance (AMR) determinants may circulate across healthcare settings, communities, animals, and the environment, highlighting the need for integrated surveillance. Although conventional urine culture remains the diagnostic gold standard, rapid molecular methods can facilitate timely diagnosis and support antimicrobial stewardship. This study analyzed 300 urine samples collected from three hospitals representing different levels of healthcare complexity in Reynosa, Tamaulipas, Mexico. Conventional urine culture and species-specific PCR assays targeting E. coli, K. pneumoniae, and P. mirabilis were performed. Fifty-six samples yielded positive urine cultures, with E. coli identified as the predominant uropathogen (85.7%, 48/56), followed by K. pneumoniae (10.7%, 6/56) and P. mirabilis (3.6%, 2/56). Positive cases were more frequent among females (70%). In addition, a multiplex PCR assay was developed and validated for the simultaneous detection of the three pathogens. The assay was also successfully applied directly to unprocessed urine samples, provided that urine represented no more than 20% of the total PCR reaction volume. The multiplex PCR demonstrated 100% concordance with conventional culture results while substantially reducing diagnostic turnaround time. To our knowledge, this is the first study conducted in Reynosa, Tamaulipas, to integrate conventional microbiological and molecular diagnostics for UTIs within a One Health framework. These findings establish a methodological baseline for regional surveillance of uropathogens at the human–healthcare–environment interface, supporting future cross-border One Health surveillance initiatives.es_ES
Doihttps://doi.org/10.66585/ohmi.2026.2.0025es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2100
Idiomaeses_ES
EditorialInfinity Integrated Publishing Centeres_ES
RelaciónOne Health Microbiology & Infectiones_ES
URL relacionadohttps://doi.org/10.66585/ohmi.2026.2.0025es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteOne Health Microbiology & Infection
TítuloMultiplex PCR for detection of bacterial uropathogens in nosocomial samples from northeastern Mexico: one health-relevant urinary tract pathogenses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorSaenz-Santos, Christian M.
AutorSotelo-Rodriguez, Anahi
AutorVillalobo-Polo, Eduardo
AutorGarcia-Oropesa, Esperanza M.
AutorEstrada-Franco, Jose G.
AutorSaenz-Santos, Christian M.es_ES
AutorSotelo-Rodriguez, Anahies_ES
AutorVillalobo-Polo, Eduardoes_ES
AutorGarcia-Oropesa, Esperanza M.es_ES
AutorEstrada-Franco, Jose G.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número1es_ES
Rango de páginas144es_ES
URL relacionadahttps://doi.org/10.66585/ohmi.2026.2.0025
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen2es_ES

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