Transcriptome and Proteome Response of Rhipicephalus annulatus Tick Vector to Babesia bigemina Infection

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:39Z
Fecha de publicación2019-01-01
ResumenA system biology approach was used to gain insight into tick biology and interactions between vector and pathogen. Rhipicephalus annulatus is one of the main vectors of Babesia bigemina which has a massive impact on animal health. It is vital to obtain more information about this relationship, to better understand tick and pathogen biology, pathogen transmission dynamics, and new potential control approaches. In ticks, salivary glands (SGs) play a key role during pathogen infection and transmission. RNA sequencing obtained from uninfected and B. bigemina infected SGs obtained from fed female ticks resulted in 6823 and 6475 unigenes, respectively. From these, 360 unigenes were found to be differentially expressed (p < 0.05). Reversed phase liquid chromatography-mass spectrometry identified a total of 3679 tick proteins. Among them 406 were differently represented in response to Babesia infection. The omics data obtained suggested that Babesia infection lead to a reduction in the levels of mRNA and proteins (n = 237 transcripts, n = 212 proteins) when compared to uninfected controls. Integrated transcriptomics and proteomics datasets suggested a key role for stress response and apoptosis pathways in response to infection. Thus, six genes coding for GP80, death-associated protein kinase (DAPK-1), bax inhibitor-1 related (BI-1), heat shock protein (HSP), heat shock transcription factor (PHSTF), and queuine trnaribosyltransferase (QtRibosyl) were selected and RNA interference (RNAi) performed. Gene silencing was obtained for all genes except phstf. Knockdown of gp80, dapk-1, and bi-1 led to a significant increase in Babesia infection levels while hsp and QtRibosyl knockdown resulted in a non-significant decrease of infection levels when compared to the respective controls. Gene knockdown did not affect tick survival, but engorged female weight and egg production were affected in the gp80, dapk-1, and QtRibosyl-silenced groups in comparison to controls. These results advanced our understanding of tick-Babesia molecular interactions, and suggested new tick antigens as putative targets for vaccination to control tick infestations and pathogen infection/transmission.es_ES
Doihttps://doi.org/10.3389/fphys.2019.00318es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5456
Idiomaenes_ES
EditorialFRONTIERS MEDIA SAes_ES
RelaciónFrontiers in Physiologyes_ES
URL relacionadohttps://doi.org/10.3389/fphys.2019.00318es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteFrontiers in Physiology
Palabra claveproteomicses_ES
Palabra clavetranscriptomicses_ES
Palabra claveRhipicephalus annulatuses_ES
Palabra claveBabesia bigeminaes_ES
Palabra clavevector-pathogen interactionses_ES
Palabra claveapoptosises_ES
Palabra clavestress responsees_ES
TítuloTranscriptome and Proteome Response of Rhipicephalus annulatus Tick Vector to Babesia bigemina Infectiones_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAntunesi, Sandra
AutorCouto, Joana
AutorFerrolho, Joana
AutorSanches, Gustavo Seron
AutorMerino Charrez, Jose Octavio
AutorDe la Cruz Hernandez, Ned
AutorMazuz, Monica
AutorVillar, Margarita
AutorShkap, Varda
Autorde la Fuentes, Jose
AutorDomingos, Ana
AutorAntunesi, Sandraes_ES
AutorCouto, Joanaes_ES
AutorFerrolho, Joanaes_ES
AutorSanches, Gustavo Serones_ES
AutorMerino Charrez, Jose Octavioes_ES
AutorDe la Cruz Hernandez, Nedes_ES
AutorMazuz, Monicaes_ES
AutorVillar, Margaritaes_ES
AutorShkap, Vardaes_ES
Autorde la Fuentes, Josees_ES
AutorDomingos, Anaes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
URL relacionadahttps://doi.org/10.3389/fphys.2019.00318
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen10es_ES

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