Gut Enzymes from Cactus Weevil (Cactophagus spinolae, Gyllenhal Coleoptera: Curculionidae) Fed Natural Diet

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:31:20Z
Fecha de publicación2014-09-01
ResumenIndian fig opuntia, Opuntia ficus-indica (L.) Mill., is a cultivated cactus adapted to semiarid conditions that has established complex interactions with insects. Several insect pests feed on and cause economic damage to the crop. The cactus weevil, Cactophagus spinolae Gyllenhal, is a phytophagous beetle that during larval and adult stages feeds on Opuntia plants, despite defensive compounds -- reported as antinutritional factors -- in the plants. Most likely the insect has developed metabolic mechanisms that enable it to use Opuntia cladodes as food, despite antinutritional factors in the plant. The aim of this work was to determine enzymes as auxiliary elements in the digestive process of the insect. The digestive enzymatic profile was described by zymographic techniques, hydrolysis of synthetic substrates, and chemical-specific inhibitors. Amylase activity was detected, represented by only one band of 80 kDa at pH 7. Serine proteinases dependent of calcium as cofactor were active at pH 8 and 9. Cysteine proteinases were active at pH 5 and 6 and found to be part of the proteolytic battery. Assays with specific substrates allowed detection of trypsin, chymotrypsin, and elastase-like proteinase activities, with 694, 1016, and 891 proteolytic activity units per milligram of protein, respectively. An attenuation effect in the activities of proteinases was detected when interacting with a protein crude extract obtained from the cladode of Indian fig opuntia, showing inhibition of the insect serine proteinases and increased cysteine proteinase activities. Interaction between plants and specialist insects is complex, and knowledge of the relationship between Opuntia plants and the insects that attack them, could be an interesting model to understand how strong the interaction is with specialist phytophagous and potentially useful to develop pest management strategies.es_ES
Doihttps://doi.org/10.3958/059.039.0309es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/3000
Idiomaenes_ES
EditorialSociety of Southwestern Entomologistses_ES
RelaciónSouthwestern Entomologistes_ES
URL relacionadohttps://doi.org/10.3958/059.039.0309es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteSouthwestern Entomologist
Palabra claveBiologyes_ES
Palabra claveCactuses_ES
Palabra claveCladodeses_ES
Palabra claveWeeviles_ES
Palabra claveTrypsines_ES
Palabra claveInsectes_ES
Palabra claveBotanyes_ES
Palabra claveDigestive enzymees_ES
Palabra claveAntinutrientes_ES
Palabra claveProteolytic enzymeses_ES
Palabra claveCurculionidaees_ES
Palabra claveAmylasees_ES
ClasificaciónBotanical Research and Applicationses_ES
TítuloGut Enzymes from Cactus Weevil (Cactophagus spinolae, Gyllenhal Coleoptera: Curculionidae) Fed Natural Dietes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAguirre-Mancilla, César Leobardo
AutorÁlvarez-Aguirre, Luis Alonso
AutorMondragón-Jacobo, C.
AutorGutiérrez-Díez, Adriana
AutorRaya-Pérez, Juan Carlos
AutorTorres‐Castillo, Jorge Ariel
AutorAguirre-Mancilla, César Leobardo
AutorÁlvarez-Aguirre, Luis Alonso
AutorMondragón-Jacobo, Candelario
AutorGutiérrez-Díez, Adriana
AutorRaya-Pérez, Juan Carlos
AutorTorres-Castillo, Jorge Ariel
AutorAguirre-Mancilla, César Leobardoes_ES
AutorÁlvarez-Aguirre, Luis Alonsoes_ES
AutorMondragón-Jacobo, C.es_ES
AutorGutiérrez-Díez, Adrianaes_ES
AutorRaya-Pérez, Juan Carloses_ES
AutorTorres‐Castillo, Jorge Arieles_ES
AutorAguirre-Mancilla, César Leobardoes_ES
AutorÁlvarez-Aguirre, Luis Alonsoes_ES
AutorMondragón-Jacobo, Candelarioes_ES
AutorGutiérrez-Díez, Adrianaes_ES
AutorRaya-Pérez, Juan Carloses_ES
AutorTorres-Castillo, Jorge Arieles_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número3es_ES
Rango de páginas477-477es_ES
URL relacionadahttps://doi.org/10.3958/059.039.0309
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen39es_ES

Files

Collections

Documentación Cientifica