Development of a rapid immunobinding test for Mycoplasma bovis cultural isolates in the genital tract of heifers
| Audiencia | Público en general | es_ES |
| Cobertura | México | es_ES |
| Fecha de ingreso | 2026-10-05T16:33:21Z | |
| Fecha de publicación | 2009-01-01 | |
| Resumen | An immunobinding test was developed on nitrocellulose paper with monoclonal antibody to diagnose Mycoplasma bovis in cultural isolates from the genital tract of artificially-infected heifers; polymerase chain reaction was used as the gold standard. The sensitivity of the immunobinding test was 5x10(3) colony-forming units, and was highly specific since there were no cross-reactions with other strains of Mycoplasma or Acholeplasma. Epidemiological sensitivity was 96.43\%, epidemiological specificity 100.00\%, positive predictive values 100.00\%, and negative predictive values were 100.00\%. There were no significant differences in diagnostic validity values between the immunobinding test and the PCR test (P>0.05). Thus, the immunobinding test is a reliable and alternative laboratory diagnostic technique for the M. bovis infection of the reproductive tract in female cattle under experimental conditions; however, it should be field tested in order to entirely evaluate the diagnostic validity of the procedure. | es_ES |
| Doi | https://doi.org/10.4081/ijas.2009.285 | es_ES |
| URI | https://riuat.uat.edu.mx/handle/123456789/5195 | |
| Idioma | en | es_ES |
| Editorial | TAYLOR \& FRANCIS LTD | es_ES |
| Relación | Italian Journal of Animal Science | es_ES |
| URL relacionado | https://doi.org/10.4081/ijas.2009.285 | es_ES |
| Derechos | Acceso abierto (Metadatos de producción científica) | es_ES |
| Licencia | http://purl.org/coar/access_right/c_abf2 | es_ES |
| Fuente | Italian Journal of Animal Science | |
| Palabra clave | Immunobinding test | es_ES |
| Palabra clave | Mycoplasma bovis | es_ES |
| Palabra clave | Genital tract | es_ES |
| Título | Development of a rapid immunobinding test for Mycoplasma bovis cultural isolates in the genital tract of heifers | es_ES |
| Tipo | Artículo | es_ES |
| Arbitrado | Ha sido Arbitrado | es_ES |
| Autor | Flores-Gutierrez, Gerardo H. | |
| Autor | Infante, Fidel | |
| Autor | Salinas-Melendez, Jose A. | |
| Autor | Thomas, Chester B. | |
| Autor | Estrada-Bellmann, Pedro C. | |
| Autor | Briones-Encinia, Florencio | |
| Autor | Flores-Gutierrez, Gerardo H. | es_ES |
| Autor | Infante, Fidel | es_ES |
| Autor | Salinas-Melendez, Jose A. | es_ES |
| Autor | Thomas, Chester B. | es_ES |
| Autor | Estrada-Bellmann, Pedro C. | es_ES |
| Autor | Briones-Encinia, Florencio | es_ES |
| Institución | Universidad Autónoma de Tamaulipas | |
| Institución | Universidad Autónoma de Tamaulipas | es_ES |
| Número | 2 | es_ES |
| Rango de páginas | 285-290 | es_ES |
| URL relacionada | https://doi.org/10.4081/ijas.2009.285 | |
| Tipo de artículo | Indexado | |
| Tipo de artículo | Indexado | es_ES |
| Volumen | 8 | es_ES |