Bioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgae

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:30:26Z
Fecha de publicación2022-01-01
ResumenSUMMARYMicroalgae have been positioned as excellent models for producing new sources of energy (biofuels and biohydrogen). Some investigations in these biological models have been directed to know if the enzymes ferredoxin (FDX) and hydrogenase (HYD) are involved in the algae producing different concentrations of molecular hydrogen (H2). To date, little is known about the concomitant transcriptional regulation of both enzymes during H2 evolution in algae. In this research, we evaluated the relative expression of hdy and fdx genes during the evolution of H2 in three microalgae (Chlorella vulgaris, Scenedesmus obliquus, and Chlamydomonas reinhardtii) in N‐deprived anaerobic cultures in the presence of Fe, and 12:24 and 24:24 h dark:light cycles. We also detected structural differences in the enzymes. The 3D modeling indicated that the 3D structure of HYD and FDX are conserved in most algal genera, and the results of our grouping according to the aa characteristics of the proteins showed two grouping trends: One, according to the algae's phylogenetic classification, and another one according to the species‐specific enzyme's characteristics, and the grouping could perhaps be more influenced by the algae's ability to produce H2. The three microalgae species reached maximum H2 accumulation values in 24h:24 h dark:light conditions in Fe‐supplemented media (4.2 ± 0.12 mL L−1 in C. vulgaris, 3.9 ± 0.10 mL L−1 in S. obliquus, and 4.5 ± 0.10 mL L−1 in C. reinhardtii), and the highest global relative expression of hyd and fdx genes was reached during the first hour of exposure to light, which suggests concomitant expression of both enzymes at the beginning of H2 production. The behavior of the expression of the hyd and fdx genes in these algal species proved to be similar between species. A better understanding of the concomitant regulation of both enzymes could lay the groundwork for the future use of both enzymes to improve H2 yields in microalgae.es_ES
Doihttps://doi.org/10.1111/pre.12500es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2392
Idiomaeses_ES
EditorialWileyes_ES
RelaciónPhycological Researches_ES
URL relacionadohttps://doi.org/10.1111/pre.12500es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuentePhycological Research
TítuloBioinformatic analysis and relative expression of hyd and fdx during H2 production in microalgaees_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorTamayo‐Ordoñez, Yahaira de Jesús
AutorAyil‐Gutiérrez, Benjamín Abraham
AutorMoreno‐Davila, Ileana Mayela Maria
AutorTamayo‐Ordoñez, Francisco Alberto
AutorCórdova‐Quiroz, Atl Victor
AutorPoot‐Poot, Wilberth Alfredo
AutorDamas‐Damas, Siprian
AutorVillanueva‐Alonzo, Hernán de Jesús
AutorTamayo‐Ordoñez, Maria Concepción
AutorTamayo‐Ordoñez, Yahaira de Jesúses_ES
AutorAyil‐Gutiérrez, Benjamín Abrahames_ES
AutorMoreno‐Davila, Ileana Mayela Mariaes_ES
AutorTamayo‐Ordoñez, Francisco Albertoes_ES
AutorCórdova‐Quiroz, Atl Victores_ES
AutorPoot‐Poot, Wilberth Alfredoes_ES
AutorDamas‐Damas, Siprianes_ES
AutorVillanueva‐Alonzo, Hernán de Jesúses_ES
AutorTamayo‐Ordoñez, Maria Concepciónes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número1es_ES
Rango de páginas37-55es_ES
URL relacionadahttps://doi.org/10.1111/pre.12500
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen71es_ES

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