Isolation and evaluation of chlorogenate hydrolase producing fungi

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:50Z
Fecha de publicación2025-01-01
ResumenChlorogenate hydrolase catalyzes the hydrolysis of chlorogenic acid, releasing caffeic and quinic acids. This enzyme can be used to extract caffeic acid from agro-industrial byproducts (apple marc, coffee pulp, brewer's spent grain, among others), and to synthesize caffeic acid derivatives. This work aimed to isolate and identify fungal strains capable of producing enzymes with chlorogenate hydrolase activity. Twelve fungal strains were isolated from fresh coffee cherries, dry coffee cherries, and green coffee beans. The isolated fungi were analyzed for their morphology and ability to assimilate chlorogenic acid. Four fungal isolates were identified as Aspergillus sp., two as Fusarium sp., and one as Cladosporium sp. All the fungal isolates were able to grow using chlorogenic acid as the sole carbon source. The CR1 strain, identified as Aspergillus niger by sequencing of the ITS region, was selected due to its high radial growth rate (0.27 ± 0.01 mm h<sup>-1</sup>). This strain grew and showed chlorogenate hydrolase activity in Submerged (SmF) and Solid-State Fermentation (SSF). The maximum chlorogenate hydrolase activity in SSF (34.6 ± 11.8 U/L) was 2.1-fold higher than in SmF (16.2 ± 1.6 U/L). These results provide the basis for developing applications based on the use of enzymes with chlorogenate hydrolase activity.es_ES
Doihttps://doi.org/10.29267/mxjb.2025.10.2.25-41es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5603
Idiomaenes_ES
EditorialUniversidad Autonoma de Tlaxcalaes_ES
RelaciónMexican Journal of Biotechnologyes_ES
URL relacionadohttps://doi.org/10.29267/mxjb.2025.10.2.25-41es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteMexican Journal of Biotechnology
Palabra claveChlorogenate hydrolasees_ES
Palabra claveChlorogenic acides_ES
Palabra claveSolid State Fermentationes_ES
Palabra claveSubmerged fermentationes_ES
TítuloIsolation and evaluation of chlorogenate hydrolase producing fungies_ES
Título alternativoAislamiento y evaluación de hongos productores de clorogenato hidrolasaes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorSoria-Ibarra, Laura Patricia
AutorPichardo-Sánchez, María Alejandra
AutorParedes-Sánchez, Francisco Alejandro
AutorBustos-Vázquez, Ma. Guadalupe
AutorRodríguez-Durán, Luis Víctor
AutorSoria-Ibarra, Laura Patriciaes_ES
AutorPichardo-Sánchez, María Alejandraes_ES
AutorParedes-Sánchez, Francisco Alejandroes_ES
AutorBustos-Vázquez, Ma. Guadalupees_ES
AutorRodríguez-Durán, Luis Víctores_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número2es_ES
Rango de páginas25-41es_ES
URL relacionadahttps://doi.org/10.29267/mxjb.2025.10.2.25-41
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen10es_ES

Files

Collections

Documentación Cientifica