Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:37Z
Fecha de publicación2009-01-01
ResumenBackground: The cattle pathogen, Anaplasma marginale, undergoes a developmental cycle in ticks that begins in gut cells. Transmission to cattle occurs from salivary glands during a second tick feeding. At each site of development two forms of A. marginale ( reticulated and dense) occur within a parasitophorous vacuole in the host cell cytoplasm. However, the role of tick genes in pathogen development is unknown. Four genes, found in previous studies to be differentially expressed in Dermacentor variabilis ticks in response to infection with A. marginale, were silenced by RNA interference ( RNAi) to determine the effect of silencing on the A. marginale developmental cycle. These four genes encoded for putative glutathione S-transferase ( GST), salivary selenoprotein M ( SelM), H+ transporting lysosomal vacuolar proton pump ( vATPase) and subolesin. Results: The impact of gene knockdown on A. marginale tick infections, both after acquiring infection and after a second transmission feeding, was determined and studied by light microscopy. Silencing of these genes had a different impact on A. marginale development in different tick tissues by affecting infection levels, the densities of colonies containing reticulated or dense forms and tissue morphology. Salivary gland infections were not seen in any of the gene-silenced ticks, raising the question of whether these ticks were able to transmit the pathogen. Conclusion: The results of this RNAi and light microscopic analyses of tick tissues infected with A. marginale after the silencing of genes functionally important for pathogen development suggest a role for these molecules during pathogen life cycle in ticks.es_ES
Doihttps://doi.org/10.1186/1471-213x-9-42es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5419
Idiomaenes_ES
EditorialBIOMED CENTRAL LTDes_ES
RelaciónBmc Developmental Biologyes_ES
URL relacionadohttps://doi.org/10.1186/1471-213x-9-42es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteBmc Developmental Biology
TítuloSilencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilises_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorKocan, Katherine M.
AutorZivkovic, Zorica
AutorBlouin, Edmour F.
AutorNaranjo, Victoria
AutorAlmazan, Consuelo
AutorMitra, Ruchira
Autorde la Fuente, Jose
AutorKocan, Katherine M.es_ES
AutorZivkovic, Zoricaes_ES
AutorBlouin, Edmour F.es_ES
AutorNaranjo, Victoriaes_ES
AutorAlmazan, Consueloes_ES
AutorMitra, Ruchiraes_ES
Autorde la Fuente, Josees_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
URL relacionadahttps://doi.org/10.1186/1471-213x-9-42
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen9es_ES

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