Intrinsic and extrinsic apoptotic pathways are involved in rat testis by cold water immersion-induced acute and chronic stress

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:37Z
Fecha de publicación2015-01-01
ResumenTesticular apoptosis is activated by stress, but it is not clear which signaling pathway is activated in response to stress. The aim of this study was to investigate whether intrinsic, extrinsic, or both apoptotic signaling pathways are activated by acute and chronic stress. Adult male rats were subjected to cold water immersion-induced stress for 1, 20, 40, and 50 consecutive days. The seminiferous tubules:apoptotic cell ratio was assayed on acute (1 day) and chronic (20, 40, 50 days) stress. Apoptotic markers, including cleaved-caspase 3 and 8, the pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins were also determined after acute and chronic stress induction. Additionally, epididymal sperm quality was evaluated, as well as corticosterone and testosterone levels. An increase in tubule apoptotic cell count percentage after an hour of acute stress and during chronic stress induction was observed. The apoptotic cells rate per tubule increment was only detected one hour after acute stress, but not with chronic stress. Accordingly, there was an increase in Bax, cleaved caspase-8 and caspase-3 proapoptotic proteins with a decrease of anti-apoptotic Bcl-2 in both acutely and chronically stressed male testes. In addition, sperm count, viability, as well as total and progressive motility were low in chronically stressed males. Finally, the levels of corticosterone increased whereas testosterone levels decreased in chronically stressed males. Activation of the extrinsic apoptotic pathway was shown by cleaved caspase-8 increase whereas the intrinsic apoptotic pathway activation was determined by the increase of Bax, along with Bcl-2 decrease, making evident a cross-talk between these two pathways with the activation of caspase-3. These results suggest that both acute and chronic stress can potentially activate the intrinsic/extrinsic apoptosis pathways in testes. Chronic stress also reduces the quality of epididymal spermatozoa, possibly due to a decrease in testosterone.es_ES
Doihttps://doi.org/10.3109/19396368.2015.1030473es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4454
Idiomaenes_ES
EditorialTAYLOR \& FRANCIS INCes_ES
RelaciónSystems Biology in Reproductive Medicinees_ES
URL relacionadohttps://doi.org/10.3109/19396368.2015.1030473es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteSystems Biology in Reproductive Medicine
Palabra claveApoptosises_ES
Palabra claveextrinsic pathwayes_ES
Palabra clavegerm cellses_ES
Palabra claveintrinsic pathwayes_ES
Palabra clavestresses_ES
Palabra clavetestises_ES
TítuloIntrinsic and extrinsic apoptotic pathways are involved in rat testis by cold water immersion-induced acute and chronic stresses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorLizbeth Juarez-Rojas, Adriana
AutorGarcia-Lorenzana, Mario
AutorAragon-Martinez, Andres
AutorEnrique Gomez-Quiroz, Luis
Autordel Socorro Retana-Marquez, Maria
AutorLizbeth Juarez-Rojas, Adrianaes_ES
AutorGarcia-Lorenzana, Marioes_ES
AutorAragon-Martinez, Andreses_ES
AutorEnrique Gomez-Quiroz, Luises_ES
Autordel Socorro Retana-Marquez, Mariaes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número4es_ES
Rango de páginas211-221es_ES
URL relacionadahttps://doi.org/10.3109/19396368.2015.1030473
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen61es_ES

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