Immunocytochemical detection of estrogen receptor-α in the female rabbit forebrain: Topography and regulation by estradiol

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:33Z
Fecha de publicación2003-01-01
ResumenTwo antibodies (H222 and Zymed) directed towards different sites of the estrogen receptor-alpha (ERalpha) were used for the following objectives: (1) to map the ERalpha in the forebrain of ovariectomized (ovx) rabbits by immunocytochemistry and (2) to determine the effect of endogenous (intact non-pregnant animals) and exogenous (ovx, estrogen-treated animals) estradiol (E2) on the population of ERalpha in the forebrain. Similar results were obtained with both antibodies used: dense aggregations of ERalpha-immunoreactive (IR) neurons were found in the infundibular nucleus (IN), the medial preoptic area (POA), the bed nucleus of the stria terminalis (BNST), and some nuclei of the amygdala. By contrast, no ERalpha-IR neurons were present in the ventromedial hypothalamic nucleus (VMN), but a dense aggregation of ERalpha-IR neurons occurred lateral to it in nucleus X. Numerous ERalpha-IR neurons were observed in the paraventricular hypothalamic nucleus, but not in the supraoptic or suprachiasmatic nuclei. The hippocampus proper lacked ERalpha-IR neurons, but the ventral subiculum in the hippocampal formation had a dense group of such cells. Intact nonpregnant rabbits showed less ERalpha-IR neurons in all regions tested than ovx animals. This difference was particularly clear in the medial POA, amygdala and BNST, while the IN showed only a marginal decrease. The dorsal, but much less the ventral, part of nucleus X also showed a decrease in the number of ERalpha-IR neurons compared with ovx animals. E2 benzoate (5 mug/day for 5 days) reduced even further the number of ERalpha-IR neurons in all regions except in a circumscribed area of the IN and the ventral part of nucleus X. These results show the existence of both sensitive and insensitive neurons to the down-regulatory effect of E2 on the presence of ERalpha. Sensitive neurons are located in the telencephalon, POA and several hypothalamic nuclei (PVN), while insensitive neurons are mainly restricted to the IN and the ventral part of nucleus X in the basal hypothalamus. Copyright (C) 2003 S. Karger AG, Basel.es_ES
Doihttps://doi.org/10.1159/000069508es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5375
Idiomaenes_ES
EditorialKARGERes_ES
RelaciónNeuroendocrinologyes_ES
URL relacionadohttps://doi.org/10.1159/000069508es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteNeuroendocrinology
Palabra clavepreoptic areaes_ES
Palabra clavelimbic systemes_ES
Palabra claveimmunocytochemistryes_ES
Palabra claveestrous cyclees_ES
Palabra claveovulationes_ES
Palabra claveparaventricular nucleuses_ES
Palabra clavegonadal steroidses_ES
Palabra clavegonadal steroid receptorses_ES
TítuloImmunocytochemical detection of estrogen receptor-α in the female rabbit forebrain: Topography and regulation by estradioles_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorCaba, M
AutorBeyer, C
AutorGonzález-Mariscal, G
AutorMorrell, JI
AutorCaba, Mes_ES
AutorBeyer, Ces_ES
AutorGonzález-Mariscal, Ges_ES
AutorMorrell, JIes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número3es_ES
Rango de páginas208-222es_ES
URL relacionadahttps://doi.org/10.1159/000069508
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen77es_ES

Files

Collections

Documentación Cientifica