Rapid and sensitive determination of levofloxacin in microsamples of human plasma by high‐performance liquid chromatography and its application in a pharmacokinetic study

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:30:31Z
Fecha de publicación2014-01-01
ResumenAbstractA rapid, sensitive and simple high‐performance liquid chromatographic assay with ultraviolet detection was developed for the quantification of levofloxacin in microsamples (100 μL) of human plasma. The extraction procedure included a protein precipitation technique and a short chromatographic running time (4.5 min). Analyses were carried out on a Symmetry C18 column using a mixture of acetonitrile and 0.01 m potassium dihydrogen aqueous solution (pH 3.4; 14:86 v/v) as mobile phase. The method provided specificity and was linear (r ≥ 0.9992) over the concentration range 0.1–12 µg/mL. The average absolute recovery was 93.59%. The intra‐ and inter‐day coefficients of variation were <6%. Additionally, levofloxacin was stable in all evaluations. The usefulness of this method was demonstrated in a pharmacokinetic study of levofloxacin in healthy adult volunteers. The present method offers two main advantages: (a) the use of microsamples reduces the total volume of blood to be collected from patients; and (b) it provides a good cost–effectiveness ratio. It is concluded that the method is rapid, simple, sensitive, economical and suitable for the determination of levofloxacin in human plasma using a small volume of sample. Copyright © 2014 John Wiley & Sons, Ltd.es_ES
Doihttps://doi.org/10.1002/bmc.3278es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2482
Idiomaeses_ES
EditorialWileyes_ES
RelaciónBiomedical Chromatographyes_ES
URL relacionadohttps://doi.org/10.1002/bmc.3278es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteBiomedical Chromatography
TítuloRapid and sensitive determination of levofloxacin in microsamples of human plasma by high‐performance liquid chromatography and its application in a pharmacokinetic studyes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAguilar‐Carrasco, José Carlos
AutorHernández‐Pineda, Jessica
AutorJiménez‐Andrade, Juan Miguel
AutorFlores‐Murrieta, Francisco Javier
AutorCarrasco‐Portugal, Miriam del Carmen
AutorLópez‐Canales, Jorge Skiold
AutorAguilar‐Carrasco, José Carloses_ES
AutorHernández‐Pineda, Jessicaes_ES
AutorJiménez‐Andrade, Juan Migueles_ES
AutorFlores‐Murrieta, Francisco Javieres_ES
AutorCarrasco‐Portugal, Miriam del Carmenes_ES
AutorLópez‐Canales, Jorge Skioldes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número3es_ES
Rango de páginas341-345es_ES
URL relacionadahttps://doi.org/10.1002/bmc.3278
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen29es_ES

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