Subolesin expression in response to pathogen infection in ticks

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:39Z
Fecha de publicación2010-01-01
ResumenBackground: Ticks (Acari: Ixodidae) are vectors of pathogens worldwide that cause diseases in humans and animals. Ticks and pathogens have co-evolved molecular mechanisms that contribute to their mutual development and survival. Subolesin was discovered as a tick protective antigen and was subsequently shown to be similar in structure and function to akirins, an evolutionarily conserved group of proteins in insects and vertebrates that controls NF-kB-dependent and independent expression of innate immune response genes. The objective of this study was to investigate subolesin expression in several tick species infected with a variety of pathogens and to determine the effect of subolesin gene knockdown on pathogen infection. In the first experiment, subolesin expression was characterized in ticks experimentally infected with the cattle pathogen, Anaplasma marginale. Subolesin expression was then characterized in questing or feeding adult ticks confirmed to be infected with Anaplasma, Ehrlichia, Rickettsia, Babesia or Theileria spp. Finally, the effect of subolesin knockdown by RNA interference (RNAi) on tick infection was analyzed in Dermacentor variabilis males exposed to various pathogens by capillary feeding (CF). Results: Subolesin expression increased with pathogen infection in the salivary glands but not in the guts of tick vector species infected with A. marginale. When analyzed in whole ticks, subolesin expression varied between tick species and in response to different pathogens. As reported previously, subolesin knockdown in D. variabilis infected with A. marginale and other tick-borne pathogens resulted in lower infection levels, while infection with Francisella tularensis increased in ticks after RNAi. When non-tick-borne pathogens were fed to ticks by CF, subolesin RNAi did not affect or resulted in lower infection levels in ticks. However, subolesin expression was upregulated in D. variabilis exposed to Escherichia coli, suggesting that although this pathogen may induce subolesin expression in ticks, silencing of this molecule reduced bacterial multiplication by a presently unknown mechanism. Conclusions: Subolesin expression in infected ticks suggested that subolesin may be functionally important for tick innate immunity to pathogens, as has been reported for the akirins. However, subolesin expression and consequently subolesin-mediated innate immunity varied with the pathogen and tick tissue. Subolesin may plays a role in tick innate immunity in the salivary glands by limiting pathogen infection levels, but activates innate immunity only for some pathogen in the guts and other tissues. In addition, these results provided additional support for the role of subolesin in other molecular pathways including those required for tissue development and function and for pathogen infection and multiplication in ticks. Consequently, RNAi experiments demonstrated that subolesin knockdown in ticks may affect pathogen infection directly by reducing tick innate immunity that resultses_ES
Doihttps://doi.org/10.1186/1471-2172-11-7es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5471
Idiomaenes_ES
EditorialBMCes_ES
RelaciónBMC Immunologyes_ES
URL relacionadohttps://doi.org/10.1186/1471-2172-11-7es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteBMC Immunology
TítuloSubolesin expression in response to pathogen infection in tickses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorZivkovic, Zorica
AutorTorina, Alessandra
AutorMitra, Ruchira
AutorAlongi, Angela
AutorScimeca, Salvatore
AutorKocan, Katherine M.
AutorGalindo, Ruth C.
AutorAlmazan, Consuelo
AutorBlouin, Edmour F.
AutorVillar, Margarita
AutorNijhof, Ard M.
AutorMani, Rinosh
AutorLa Barbera, Giuseppa
AutorCaracappa, Santo
AutorJongejan, Frans
Autorde la Fuente, Jose
AutorZivkovic, Zoricaes_ES
AutorTorina, Alessandraes_ES
AutorMitra, Ruchiraes_ES
AutorAlongi, Angelaes_ES
AutorScimeca, Salvatorees_ES
AutorKocan, Katherine M.es_ES
AutorGalindo, Ruth C.es_ES
AutorAlmazan, Consueloes_ES
AutorBlouin, Edmour F.es_ES
AutorVillar, Margaritaes_ES
AutorNijhof, Ard M.es_ES
AutorMani, Rinoshes_ES
AutorLa Barbera, Giuseppaes_ES
AutorCaracappa, Santoes_ES
AutorJongejan, Franses_ES
Autorde la Fuente, Josees_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
URL relacionadahttps://doi.org/10.1186/1471-2172-11-7
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen11es_ES

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