Diversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistance

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:30:36Z
Fecha de publicación2009-12-11
ResumenThe objective of this study was to determine the distribution of staphylococcal cassette chromosome mec (SCCmec) elements in meticillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from a tertiary-care hospital in Mexico and to examine the relationship to drug resistance. Fifty selected MR-CoNS isolates collected from catheters (n=15), blood (n=15), bone (n=9), bronchial lavage (n=2) and urine (n=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion, synovial fluid, tracheal aspirate and wound secretion were examined. Susceptibility testing was performed by the broth microdilution method. SCCmec types were determined by multiplex PCR and PFGE was carried out as described previously for Staphylococcus aureus. Among the MR-CoNS strains studied, the most frequently isolated species were Staphylococcus epidermidis (n=26) and Staphylococcus haemolyticus (n=13). Staphylococcus cohnii (n=5), Staphylococcus hominis (n=3), Staphylococcus sciuri (n=1), Staphylococcus pasteuri (n=1) and the recently described species Staphylococcus pettenkoferi (n=1) were also identified. The most frequent MR-CoNS genotype identified was SCCmec type IVa in S. epidermidis isolates, which also showed a high diversity in their PFGE patterns. A clone was found that amplified both SCCmec III and V elements in five isolates examined. The single MR S. pettenkoferi isolate harboured SCCmec type IVd and the single MR S. pasteuri isolate harboured SCCmec type I. The carriage of SCCmec type III was associated with resistance or intermediate resistance to meropenem (P <0.05). These results confirm the high prevalence of S. epidermidis SCCmec IVa and the high genetic diversity among MR-CoNS strains. As far as is known, this is the first report describing the newly identified S. pettenkoferi possessing SCCmec IVd and S. pasteuri harbouring SCCmec type I. MR-CoNS harbouring SCCmec type III were found to be more resistant to meropenem.es_ES
Doihttps://doi.org/10.1099/jmm.0.015800-0es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2548
Idiomaenes_ES
EditorialMicrobiology Societyes_ES
RelaciónJournal of Medical Microbiologyes_ES
URL relacionadohttps://doi.org/10.1099/jmm.0.015800-0es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteJournal of Medical Microbiology
Palabra claveCoagulasees_ES
Palabra claveMicrobiologyes_ES
Palabra claveDrug resistancees_ES
Palabra claveBiologyes_ES
Palabra claveStaphylococcuses_ES
Palabra claveGeneticses_ES
Palabra claveStaphylococcus haemolyticuses_ES
Palabra claveDiversity (politics)es_ES
Palabra claveChromosomees_ES
Palabra claveStaphylococcus aureuses_ES
Palabra claveBacteriaes_ES
Palabra claveGenees_ES
ClasificaciónAntimicrobial Resistance in Staphylococcuses_ES
TítuloDiversity of staphylococcal cassette chromosome mec structures in coagulase-negative staphylococci and relationship to drug resistancees_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorGarza‐González, Elvira
AutorLópez, Daniel
AutorPezina, Cesar
AutorMuruet-Gutierrez, Walter
AutorBocanegra‐García, Virgilio
AutorMuñoz, I. Sebastián
AutorRamírez, Camilo
AutorLlaca-Díaz, Jorge
AutorGarza‐González, Elviraes_ES
AutorLópez, Danieles_ES
AutorPezina, Cesares_ES
AutorMuruet-Gutierrez, Walteres_ES
AutorBocanegra‐García, Virgilioes_ES
AutorMuñoz, I. Sebastiánes_ES
AutorRamírez, Camiloes_ES
AutorLlaca-Díaz, Jorgees_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número3es_ES
Rango de páginas323-329es_ES
URL relacionadahttps://doi.org/10.1099/jmm.0.015800-0
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen59es_ES

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