Effect of parasite burden on the detection of Fasciola hepatica antigens in sera and feces of experimentally infected sheep

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:56Z
Fecha de publicación2001-01-01
ResumenThe effect of Fasciola hepatica parasite burden on the detection of excretory/secretory (E/S) antigens in sera and feces of experimentally infected sheep was evaluated using a double antibody-based capture enzyme-linked immunosorbent assay (ELISA). Four groups of five sheep each were used. The first three groups were infected with 50, 100 and 200 metacercariae of F. hepatica, and the fourth group remained as non-infected control. On the day of infection and weekly thereafter, serum and fecal samples were taken. ELISA detected F. hepatica E/S antigen levels in serum from the first week post-infection (wpi) and in fecal supernatant from the fourth wpi, which were significantly (p < 0.05) higher than controls. F. hepatica eggs were not detected until after the eighth wpi, The correlation between absorbance of E/S antigens in serum with the fluke burden was 0.77 (p < 0.0001) and in feces 0.76 (p < 0.0001) at 12th wpi. The sensitivity of the assay to detect E/S antigens in serum was 86.6\% and in feces 93.3\%. It is concluded that the ELISA technique used in this study offers a diagnostic alternative for detecting early infections of F. hepatica in sheep. (C) 2001 Elsevier Science B.V. All rights reserved.es_ES
Doihttps://doi.org/10.1016/s0304-4017(01)00376-4es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4787
Idiomaenes_ES
EditorialELSEVIER SCIENCE BVes_ES
RelaciónVeterinary Parasitologyes_ES
URL relacionadohttps://doi.org/10.1016/s0304-4017(01)00376-4es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteVeterinary Parasitology
Palabra claveFasciola hepaticaes_ES
Palabra claveserumes_ES
Palabra clavefeceses_ES
Palabra claveE/S antigenses_ES
Palabra claveELISAes_ES
Palabra clavesheepes_ES
TítuloEffect of parasite burden on the detection of Fasciola hepatica antigens in sera and feces of experimentally infected sheepes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAlmazán, C
AutorAvila, G
AutorQuiroz, H
AutorIbarra, F
AutorOchoa, P
AutorAlmazán, Ces_ES
AutorAvila, Ges_ES
AutorQuiroz, Hes_ES
AutorIbarra, Fes_ES
AutorOchoa, Pes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número2es_ES
Rango de páginas101-112es_ES
URL relacionadahttps://doi.org/10.1016/s0304-4017(01)00376-4
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen97es_ES

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