Fractionation and Extraction of Cell Wall Proteins From Candida albicans

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:16Z
Fecha de publicación2025-01-01
ResumenCandida albicans is the pathogenic fungus that most frequently causes infections in humans. It is part of the microbiota commonly found in the skin, gastrointestinal tract, and vaginal mucosa. However, certain conditions, including immunosuppression, excessive use of antibiotics, hormonal changes, the use of medical devices in patients, and individual nutritional status, promote the development of opportunistic infections caused by this fungus. One of the main fungal structures interacting with the host is the cell wall, which is principally composed of chitin, glucan, and proteins. The cell wall plays key functions for the cell, such as osmotic protection; it is also responsible for cellular shape and acts as a signaling hub in response to environmental changes. Cell wall proteins participate in diverse cellular functions, such as attachment to surfaces and cell wall structure; some possess catalytic or transport activities. In this protocol, we show the methodology for isolating cell wall proteins covalently linked or not to cell wall components that can be previously labeled with {[}14C]-L-lysine by the action of the fungal transglutaminase localized in the cell wall. We use an extraction method by mechanical cell disruption and washing with 2 M NaCl, whose ionic strength eliminates contaminating proteins from other organelles, through subsequent serial treatments with SDS, chitinase, and zymolyase.es_ES
Doihttps://doi.org/10.21769/bioprotoc.5456es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5104
Idiomaenes_ES
EditorialBIO-PROTOCOLes_ES
RelaciónBio-Protocoles_ES
URL relacionadohttps://doi.org/10.21769/bioprotoc.5456es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteBio-Protocol
Palabra claveCell fractionationes_ES
Palabra claveCell wall proteinses_ES
Palabra claveCandida albicanses_ES
Palabra claveCandidiasises_ES
Palabra claveTransglutaminase activityes_ES
TítuloFractionation and Extraction of Cell Wall Proteins From Candida albicanses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorReyna-Beltran, Elizabeth
AutorIranzo, Maria
AutorMormeneo, Salvador
AutorBazan-Mendez, Cesar Isaac
AutorLabra-Barrios, Maria Luisa
AutorHernandez-Martinez, Edgar
AutorLuna-Arias, Juan Pedro
AutorReyna-Beltran, Elizabethes_ES
AutorIranzo, Mariaes_ES
AutorMormeneo, Salvadores_ES
AutorBazan-Mendez, Cesar Isaaces_ES
AutorLabra-Barrios, Maria Luisaes_ES
AutorHernandez-Martinez, Edgares_ES
AutorLuna-Arias, Juan Pedroes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número18es_ES
URL relacionadahttps://doi.org/10.21769/bioprotoc.5456
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen15es_ES

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