Increased expression of FAK isoforms as potential cancer biomarkers in ovarian cancer

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:06Z
Fecha de publicación2019-01-01
ResumenFocal adhesion kinase (FAK) is a non-receptor tyrosine kinase that is expressed in most human cell types (example: Epithelial cells, fibroblasts and endothelial), it serves a key role in the control of cell survival, proliferation and motility. The abnormal expression of FAK has been associated with poor prognosis in cancer, including ovarian cancer. However, although FAK isoforms with specific molecular and functional properties have been characterized, there are a limited number of published studies that examine FAK isoforms in ovarian cancer. The aim of the present study was to analyze the expression level of FAK and its isoforms in ovarian cancer. The expression of FAK kinase and focal adhesion targeting (FAT) domains was determined with immunohistochemistry in healthy ovary, and serous and mucinous cystadenoma, borderline tumor and carcinoma samples. Additionally, the expression of FAK and its isoforms were investigated in three ovarian cancer-derived cell lines with western blotting and reverse transcription-semi-quantitative polymerase chain reaction. An increased expression of FAK kinase domain was determined in serous tumor samples and was associated with advancement of the lesion. FAK kinase domain expression was moderate-to-low in mucinous tumor samples. The expression of the FAK FAT domain in tumor samples was reduced, compared with healthy ovary samples; however, the FAT domain was localized to the cellular nucleus. Expression of alternative transcripts FAK degrees, FAK(28,6) and FAK(28) was determined in all three cell lines investigated. In conclusion, FAK kinase and FAT domains are differentially expressed among ovarian tumor types. These results indicated the presence of at least two isoforms of FAK (FAK and the putative FAK-related non-kinase) in tumor tissue, which is supported by the cells producing at least three FAK alternative transcripts. These results may support the use of FAK and its isoforms as biomarkers for ovarian cancer.es_ES
Doihttps://doi.org/10.3892/ol.2019.10147es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4917
Idiomaenes_ES
EditorialSPANDIDOS PUBL LTDes_ES
RelaciónOncology Letterses_ES
URL relacionadohttps://doi.org/10.3892/ol.2019.10147es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteOncology Letters
Palabra claveisoformses_ES
Palabra clavefocal adhesion kinasees_ES
Palabra clavebiomarkerses_ES
Palabra claveovarian canceres_ES
Palabra clavemucinouses_ES
Palabra claveserouses_ES
TítuloIncreased expression of FAK isoforms as potential cancer biomarkers in ovarian canceres_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorNolasco-Quiroga, Manuel
AutorRosas-Diaz, Marisol
AutorMoreno, Jose
AutorGodinez-Aguilar, Ricardo
AutorJose Lopez-Ibarra, Maria
AutorPina-Sanchez, Patricia
AutorAlvarado-Cabrero, Isabel
AutorVazquez-Gomez, Gerardo
AutorRocha-Zavaleta, Leticia
AutorArenas-Aranda, Diego
AutorSalamanca-Gomez, Fabio
AutorNolasco-Quiroga, Manueles_ES
AutorRosas-Diaz, Marisoles_ES
AutorMoreno, Josees_ES
AutorGodinez-Aguilar, Ricardoes_ES
AutorJose Lopez-Ibarra, Mariaes_ES
AutorPina-Sanchez, Patriciaes_ES
AutorAlvarado-Cabrero, Isabeles_ES
AutorVazquez-Gomez, Gerardoes_ES
AutorRocha-Zavaleta, Leticiaes_ES
AutorArenas-Aranda, Diegoes_ES
AutorSalamanca-Gomez, Fabioes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número6es_ES
Rango de páginas4779-4786es_ES
URL relacionadahttps://doi.org/10.3892/ol.2019.10147
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen17es_ES

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