Effects of repeated electroejaculations on kinematic sperm subpopulations and quality markers of Mexican creole goats

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:42Z
Fecha de publicación2015-01-01
ResumenHere we show the effects of repeated electroejaculation (EE) on mean values of motility, mitochondrial functionality, and expression of active caspases on goat sperm obtained by EE. Evaluations were done using CASA and flow cytometry. A strategy for identification of kinematic sperm subpopulations, when individual data of sperm are not provided by the CASA system, is provided. Fifty semen samples, five of each of ten adult creole goats, were obtained by electroejaculation. Mean values of total motility, progressive motility and flow cytometry evaluations were compared among EEs. Relationships among mean values of variables were investigated using Spearman correlation and principal component analysis (PCA). For identification of kinematic sperm subpopulations, PCA followed by hierarchical clustering was applied on data of the intervals provided automatically by the CASA system. Total motility does no change after repeated EE. Mean values of motility parameters and molecular markers were unrelated in multivariate space, but bivariate correlations were found. Values in upper and lower intervals defined clearly the sperm subpopulations, which had motility parameters changing overtime. Taken together, our results show that repeated EE does not affect mean values of total motility, that molecular markers are not related with motility parameters, and that it is possible to identify kinematic sperm subpopulations when individual data, of motility parameters, are not provided by the CASA system. (C) 2014 The Authors. Published by Elsevier B.V.es_ES
Doihttps://doi.org/10.1016/j.anireprosci.2014.12.009es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4535
Idiomaenes_ES
EditorialELSEVIERes_ES
RelaciónAnimal Reproduction Sciencees_ES
URL relacionadohttps://doi.org/10.1016/j.anireprosci.2014.12.009es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteAnimal Reproduction Science
Palabra claveComputer assisted semen analysises_ES
Palabra claveElectroejaculationes_ES
Palabra claveGoates_ES
Palabra claveFlow cytometryes_ES
Palabra claveMultivariate graphicses_ES
Palabra claveMultivariate statisticses_ES
TítuloEffects of repeated electroejaculations on kinematic sperm subpopulations and quality markers of Mexican creole goatses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorVazquez, A. J. F.
AutorCedillo, M. J.
AutorQuezada, V. J.
AutorRivas, A. C.
AutorMorales, E. C. L.
AutorAyala, E. M. E.
AutorHernandez, M. J.
AutorGonzalez, R. A.
AutorAragon, M. A.
AutorVazquez, A. J. F.es_ES
AutorCedillo, M. J.es_ES
AutorQuezada, V. J.es_ES
AutorRivas, A. C.es_ES
AutorMorales, E. C. L.es_ES
AutorAyala, E. M. E.es_ES
AutorHernandez, M. J.es_ES
AutorGonzalez, R. A.es_ES
AutorAragon, M. A.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Rango de páginas29-38es_ES
URL relacionadahttps://doi.org/10.1016/j.anireprosci.2014.12.009
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen154es_ES

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