Reverse transcription loop-mediated isothermal amplification has a high performance in the detection of SARS-CoV-2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:54Z
Fecha de publicación2023-01-01
ResumenThe detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43\% were symptomatic positive individuals, 25\% were asymptomatic positive individuals and 32\% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5\% and a specificity of 97.6\% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100\% and a specificity of 97.6\% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring.es_ES
Doihttps://doi.org/10.3892/etm.2023.12097es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4710
Idiomaenes_ES
EditorialSPANDIDOS PUBL LTDes_ES
RelaciónExperimental and Therapeutic Medicinees_ES
URL relacionadohttps://doi.org/10.3892/etm.2023.12097es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteExperimental and Therapeutic Medicine
Palabra clavesevere acute respiratory syndrome coronavirus 2es_ES
Palabra clavereverse transcription loop-mediated isothermal amplificationes_ES
Palabra claveasymptomatices_ES
Palabra clavesymptomatices_ES
Palabra clavesalivaes_ES
Palabra clavenasal swabes_ES
TítuloReverse transcription loop-mediated isothermal amplification has a high performance in the detection of SARS-CoV-2 in saliva samples and nasal swabs from asymptomatic and symptomatic individualses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorReyes-Morales, Rodolfo
AutorSegundo-Ibanez, Patricia
AutorFlores-de los Angeles, Cesar
AutorVizcarra-Ramos, David
AutorIbanez-Galeana, Damian Inaki
AutorSalas-Cuevas, Gabriela
AutorOlvera-Serrano, Angel
AutorPerez-Silva, Nancy Bibiana
AutorRocha-Rocha, Valeria Magali
AutorEl-Kassi, Elie Girgis
AutorEscobedo-Straffon, Jorge
AutorContreras-Mioni, Laura
AutorRosas-Diaz, Marisol
AutorLopez-Martinez, Karla Maria
AutorArias-Matus, Carlos Eduardo
AutorBautista-Rodriguez, Elizabeth
AutorNolasco-Quiroga, Manuel
AutorReyes-Morales, Rodolfoes_ES
AutorSegundo-Ibanez, Patriciaes_ES
AutorFlores-de los Angeles, Cesares_ES
AutorVizcarra-Ramos, Davides_ES
AutorIbanez-Galeana, Damian Inakies_ES
AutorSalas-Cuevas, Gabrielaes_ES
AutorOlvera-Serrano, Angeles_ES
AutorPerez-Silva, Nancy Bibianaes_ES
AutorRocha-Rocha, Valeria Magalies_ES
AutorEl-Kassi, Elie Girgises_ES
AutorEscobedo-Straffon, Jorgees_ES
AutorContreras-Mioni, Lauraes_ES
AutorRosas-Diaz, Marisoles_ES
AutorLopez-Martinez, Karla Mariaes_ES
AutorArias-Matus, Carlos Eduardoes_ES
AutorBautista-Rodriguez, Elizabethes_ES
AutorNolasco-Quiroga, Manueles_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número2es_ES
URL relacionadahttps://doi.org/10.3892/etm.2023.12097
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen26es_ES

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