Development and Validation of an Immunohistochemical Method for Diagnosis of Bovine Tuberculosis in Formalin-Fixed, Paraffin-Embedded Tissues

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:08Z
Fecha de publicación2012-01-01
ResumenThe objective of the study was to develop an indirect Immunoperoxidase Method for bovine tuberculosis diagnosis in formalin fixed, paraffin embedded tissues and its validation compared to mycobacterial isolation and Ziehl Neelsen staining. About of 33 bovine lymph nodes with isolation of Mycobacterium bovis and 11 negative lymph nodes from tuberculosis free ranches were used. Sections of all lymph nodes examined were stained with Ziehl Neelsen and Immunohistochemistry (IHC). Rabbit anti-M. bovis polyclonal antibodies and horse anti-rabbit were used as primary and secondary antibodies. The immunologic reaction was detected with an immunoperoxidase DAB Method and cotuaterstained with hematoxylin. Results show complete agreement between Immunohistochemistry and mycobacterial culture. From the 33 positive isolation cases, all of them (100\%) were positive by IHC. From the 11 negative cases, all of them were negative to Mycobacterium by IHC. Regarding Ziehl Neelsen of the 33 positive isolation cases, 30 (90.9\%) had acid-fast bacilli and from the 11 negative isolation cases none had acid fast bacilli. Results show that IHC represents a fast, sensitive and specific diagnostic tool for bovine tuberculosis in formalin fixed, paraffin embedded tissues allowing simultaneous observation of tissue lesions and antigens.es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4985
Idiomaenes_ES
EditorialMEDWELL ONLINEes_ES
RelaciónJournal of Animal and Veterinary Advanceses_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteJournal of Animal and Veterinary Advances
Palabra claveBovinees_ES
Palabra claveformalin fixedes_ES
Palabra claveimmunohistochemistryes_ES
Palabra clavetuberculosises_ES
Palabra claveantibodieses_ES
TítuloDevelopment and Validation of an Immunohistochemical Method for Diagnosis of Bovine Tuberculosis in Formalin-Fixed, Paraffin-Embedded Tissueses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorMartinez-Burnes, J.
AutorCastillo-Martinez, O.
AutorSolano-Terreros, D.
Autorde la Cruz-Hernandez, N. I.
AutorCampuzano-Granados, J.
AutorBarrios-Garcia, H.
AutorSnydelaar Hardwicke, A.
AutorAlmazan-Garcia, C.
AutorGuizarnotegui-Blanco, J. A.
AutorRamos-Vara, J.
AutorMartinez-Burnes, J.es_ES
AutorCastillo-Martinez, O.es_ES
AutorSolano-Terreros, D.es_ES
Autorde la Cruz-Hernandez, N. I.es_ES
AutorCampuzano-Granados, J.es_ES
AutorBarrios-Garcia, H.es_ES
AutorSnydelaar Hardwicke, A.es_ES
AutorAlmazan-Garcia, C.es_ES
AutorGuizarnotegui-Blanco, J. A.es_ES
AutorRamos-Vara, J.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número16es_ES
Rango de páginas2856-2860es_ES
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen11es_ES

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