Characterization of Mechanical Allodynia and Skin Innervation in a Mouse Model of Type-2 Diabetes Induced by Cafeteria-Style Diet and Low-Doses of Streptozotocin

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:02Z
Fecha de publicación2021-01-01
ResumenBackground: Painful distal symmetrical polyneuropathy (DPN) is a frequent complication of type-2 diabetes mellitus (T2DM) that commonly presents as neuropathic pain and loss of skin nerve fibers. However, there are limited therapies to effectively treat DPN and many of the current animal models of T2DM-induced DPN do not appear to mirror the human disease. Thus, we validated a DPN mouse model induced by a cafeteria-style diet plus low-doses of streptozotocin (STZ). Methods: Female C57BL/6J mice were fed either standard (STD) diet or obesogenic cafeteria (CAF) diet for 32 weeks, starting at 8 weeks old. Eight weeks after starting diets, CAF or STD mice received either four low-doses of STZ or vehicle. Changes in body weight, blood glucose and insulin levels, as well as oral glucose- and insulin-tolerance tests (OGTT and ITT) were determined. The development of mechanical hypersensitivity of the hindpaws was determined using von Frey filaments. Moreover, the effect of the most common neuropathic pain drugs was evaluated on T2DM-induced mechanical allodynia. Finally, the density of PGP -9.5(+) (a pan-neuronal marker) axons in the epidermis from the hindpaw glabrous skin was quantified. Results: At 22-24 weeks after STZ injections, CAF + STZ mice had significantly higher glucose and insulin levels compared to CAF + VEH, STD + STZ, and STD + VEH mice, and developed glucose tolerance and insulin resistance. Skin mechanical sensitivity was detected as early as 12 weeks post-STZ injections and it was significantly attenuated by intraperitoneal acute treatment with amitriptyline, gabapentin, tramadol, duloxetine, or carbamazepine but not by diclofenac. The density of PGP-9.5(+) nerve fibers was reduced in CAF + STZ mice compared to other groups. Conclusion: This reverse translational study provides a painful DPN mouse model which may help in developing a better understanding of the factors that generate and maintain neuropathic pain and denervation of skin under T2DM and to identify mechanism-based new treatments.es_ES
Doihttps://doi.org/10.3389/fphar.2020.628438es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4870
Idiomaenes_ES
EditorialFRONTIERS MEDIA SAes_ES
RelaciónFrontiers in Pharmacologyes_ES
URL relacionadohttps://doi.org/10.3389/fphar.2020.628438es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteFrontiers in Pharmacology
Palabra clavepainful distal polyneuropathyes_ES
Palabra clavetype 2 diabetes mellituses_ES
Palabra claveskin hypersensitivityes_ES
Palabra clavemechanical allodyniaes_ES
Palabra claveskin nerve fiberses_ES
Palabra clavecafeteria dietes_ES
TítuloCharacterization of Mechanical Allodynia and Skin Innervation in a Mouse Model of Type-2 Diabetes Induced by Cafeteria-Style Diet and Low-Doses of Streptozotocines_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorCastaneda-Corral, Gabriela
AutorVelazquez-Salazar, Norma B.
AutorMartinez-Martinez, Arisai
AutorTaboada-Serrano, Juanita N.
AutorNunez-Aragon, Pablo N.
AutorGonzalez-Palomares, Laura
AutorIssel Acosta-Gonzalez, Rosa
AutorPetricevich, Vera L.
AutorJose Acevedo-Fernandez, Juan
AutorMontes, Sergio
AutorMiguel Jimenez-Andrade, Juan
AutorCastaneda-Corral, Gabrielaes_ES
AutorVelazquez-Salazar, Norma B.es_ES
AutorMartinez-Martinez, Arisaies_ES
AutorTaboada-Serrano, Juanita N.es_ES
AutorNunez-Aragon, Pablo N.es_ES
AutorGonzalez-Palomares, Lauraes_ES
AutorIssel Acosta-Gonzalez, Rosaes_ES
AutorPetricevich, Vera L.es_ES
AutorJose Acevedo-Fernandez, Juanes_ES
AutorMontes, Sergioes_ES
AutorMiguel Jimenez-Andrade, Juanes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
URL relacionadahttps://doi.org/10.3389/fphar.2020.628438
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen11es_ES

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