The leucokinin-like peptide receptor from the cattle fever tick, Rhipicephalus microplus, is localized in the midgut periphery and receptor silencing with validated double-stranded RNAs causes a reproductive fitness cost

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:38Z
Fecha de publicación2019-01-01
ResumenThe cattle fever tick, Rhipicephalus microplus (Canestrini) (Acari: Ixodidae), is a one-host tick that infests primarily cattle in tropical and sub-tropical regions of the world. This species transmits deadly cattle pathogens, especially Babesia spp., for which a recombinant vaccine is not available. Therefore, disease control depends on tick vector control. Although R. microplus was eradicated in the USA, tick populations in Mexico and South America have acquired resistance to many of the applied acaricides. Recent acaricide-resistant tick reintroductions detected in the U.S. underscore the need for novel tick control methods. The octopamine and tyramine/octopamine receptors, both G protein-coupled receptors (GPCR), are believed to be the main molecular targets of the acaricide amitraz. This provides the proof of principle that investigating tick GPCRs, especially those that are invertebrate-specific, may be a feasible strategy for discovering novel targets and subsequently new anti-tick compounds. The R. microplus leucokinin-like peptide receptor (LKR), also known as the myokinin- or kinin receptor, is such a GPCR. While the receptor was previously characterized in vitro, the function of the leucokinin signaling system in ticks remains unknown. In this work, the LKR was immunolocalized to the periphery of the female midgut and silenced through RNA interference (RNAi) in females. To optimize RNAi experiments, a dual-luciferase system was developed to determine the silencing efficiency of LKR-double stranded RNA (dsRNA) constructs prior to testing those in ticks placed on cattle. This assay identified two effective dsRNAs. Silencing of the LKR with these two validated dsRNA constructs was verified by quantitative real time PCR (qRT-PCR) of female tick dissected tissues. Silencing was significant in midguts and carcasses. Silencing caused decreases in weights of egg masses and in the percentages of eggs hatched per egg mass, as well as delays in time to oviposition and egg hatching. A role of the kinin receptor in tick reproduction is apparent. (C) 2019 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.es_ES
Doihttps://doi.org/10.1016/j.ijpara.2018.11.006es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5449
Idiomaenes_ES
EditorialELSEVIER SCI LTDes_ES
RelaciónInternational Journal for Parasitologyes_ES
URL relacionadohttps://doi.org/10.1016/j.ijpara.2018.11.006es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteInternational Journal for Parasitology
Palabra claveRhipicephalus micropluses_ES
Palabra claveKinin GPCRes_ES
Palabra claveMidgut immunolocalizationes_ES
Palabra claveReproductiones_ES
Palabra claveDual luciferase reporter systemes_ES
Palabra claveRNAies_ES
TítuloThe leucokinin-like peptide receptor from the cattle fever tick, Rhipicephalus microplus, is localized in the midgut periphery and receptor silencing with validated double-stranded RNAs causes a reproductive fitness costes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorBrock, Christina M.
AutorTemeyer, Kevin B.
AutorTidwell, Jason
AutorYang, Yunlong
AutorBlandon, Maria A.
AutorCarreon-Camacho, Diana
AutorLongnecker, Michael T.
AutorAlmazan, Consuelo
Autorde Leon, Adalberto A. Perez
AutorPietrantonio, Patricia V.
AutorBrock, Christina M.es_ES
AutorTemeyer, Kevin B.es_ES
AutorTidwell, Jasones_ES
AutorYang, Yunlonges_ES
AutorBlandon, Maria A.es_ES
AutorCarreon-Camacho, Dianaes_ES
AutorLongnecker, Michael T.es_ES
AutorAlmazan, Consueloes_ES
Autorde Leon, Adalberto A. Perezes_ES
AutorPietrantonio, Patricia V.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número3-4es_ES
Rango de páginas287-299es_ES
URL relacionadahttps://doi.org/10.1016/j.ijpara.2018.11.006
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen49es_ES

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