Inhibition of modori-associated proteinases by legume seed extracts in surimi production

Abstract

The modori phenomenon from several fish species has been associated with endogenous; proteinolytic enzymes. We evaluated the presence of modori-associated proteinases in Mexican flounder and Atlantic croaker, and the decrease of the modori effect by inhibition of the responsible enzymes with serine-proteinase inhibitors from legume seeds. The presence of modori was evaluated by changes in shear stress and shear strain at failure in surimi gels incubated for 30 min at 60degreesC. Modori was associated with proteolytic degradation of myosin by SDS-PAGE analysis. Crude extracts from kidney bean, pea, chickpea, lentil, and soybean inhibited proteolytic activities of both fish to different extents.