Melimine-Modified 3D-Printed Polycaprolactone Scaffolds for the Prevention of Biofilm-Related Biomaterial Infections

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:39Z
Fecha de publicación2022-01-01
ResumenBiomaterial-associated infections are one of the major causes of implant failure. These infections result from persistent bacteria that have adhered to the biomaterial surface before, during, or after surgery and have formed a biofilm on the implant's surface. It is estimated that 4 to 10\% of implant surfaces are contaminated with bacteria; however, the infection rate can be as h i g h as 30\% in intensive care units in developed countries and as h i g h as 45\% in developing countries. To date, there is no clinical solution to prevent implant infection without relying on the use of high doses of antibiotics supplied systemically and/or removal of the infected device. In this study, melimine, a chimeric cationic peptide that has been tested in Phase I and II human clinical trials, was immobilized onto the surface of 3D-printed medical-grade polycaprolactone (mPCL) scaffolds via covalent binding and adsorption. X-ray photoelectron spectroscopy (XPS) and time-of-fl i g h t secondary ion mass spectrometry (ToF-SIMS) spectra of melimine-treated surfaces confirmed immobilization of the peptide, as wel l as its homogeneous distribution throughout the scaffold surface. Amino acid analysis showed that melimine covalent and noncovalent immobilization resulted in a peptide density of similar to 156 and similar to 533 ng/cm(2) , respectively. Furthermore, we demonstrated that the immobilization of melimine on mPCL scaffolds by 1-ethyl-3-{[}3-(dimethylamino)propyl] carbodiimide hydrochloride (EDC) coupling and noncovalent interactions resulted in a reduction of Staphylococcus aureus colonization by 78.7\% and 76.0\%, respectiv e l y , in comparison with the nonmodified control specimens. Particularly, the modified surfaces maintained their antibacterial properties for 3 days, which resulted in the inhibition of biofilm formation in vitro. This system offers a biomaterial strategy to effectively prevent biofilm-related infections on implant surfaces without relying on the use of prophylactic antibiot i c treatment.es_ES
Doihttps://doi.org/10.1021/acsnano.2c05812es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5460
Idiomaenes_ES
EditorialAMER CHEMICAL SOCes_ES
RelaciónACS Nanoes_ES
URL relacionadohttps://doi.org/10.1021/acsnano.2c05812es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteACS Nano
Palabra clavebacterial infectiones_ES
Palabra claveantimicrobial peptidees_ES
Palabra clavepolycaprolactonees_ES
Palabra clave3D printinges_ES
Palabra clavescaffoldes_ES
Palabra clavemeliminees_ES
TítuloMelimine-Modified 3D-Printed Polycaprolactone Scaffolds for the Prevention of Biofilm-Related Biomaterial Infectionses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorCometta, Silvia
AutorJones, Robert T.
AutorJuarez-Saldivar, Alfredo
AutorDonose, Bogdan C.
AutorYasir, Muhammad
AutorBock, Nathalie
AutorDargaville, Tim R.
AutorBertling, Karl
AutorBruenig, Michael
AutorRakic, Aleksandar D.
AutorWillcox, Mark
AutorHutmacher, Dietmar W.
AutorCometta, Silviaes_ES
AutorJones, Robert T.es_ES
AutorJuarez-Saldivar, Alfredoes_ES
AutorDonose, Bogdan C.es_ES
AutorYasir, Muhammades_ES
AutorBock, Nathaliees_ES
AutorDargaville, Tim R.es_ES
AutorBertling, Karles_ES
AutorBruenig, Michaeles_ES
AutorRakic, Aleksandar D.es_ES
AutorWillcox, Markes_ES
AutorHutmacher, Dietmar W.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número10es_ES
Rango de páginas16497-16512es_ES
URL relacionadahttps://doi.org/10.1021/acsnano.2c05812
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen16es_ES

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