Stable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplast

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:57Z
Fecha de publicación2015-01-01
ResumenBackground: The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidase (MnP-2 gene) from the chloroplast genome. Results: pES4 and pES5 vectors were derived from pPV111A plasmid and contain the PelA and MnP-2 synthetic genes, respectively. Both genes are flanked by a synthetic rrn16S promoter and the 3'UTR from rbcL gene. Efficient gene integration into both inverted repeats of the intergenic region between rrn16S and 3'rps'12 was confirmed by Southern blot. Stable processing and expression of the RNA were confirmed by Northern blot analysis. Enzymatic activity was evaluated to detect expression and functionality of both enzymes. In general, mature plants showed more activity than young transplastomic plants. Compared to wild type plants, transplastomic events expressing pectin lyase exhibited enzymatic activity above 58.5\% of total soluble protein at neutral pH and 60 degrees C. In contrast, MnP-2 showed high activity at pH 6 with optimum temperature at 65 degrees C. Neither transplastomic plant exhibited an abnormal phenotype. Conclusion: This study demonstrated that hydrolytic genes PelA and MnP-2 could be integrated and expressed correctly from the chloroplast genome of tobacco plants. A whole plant, having similar to 470 g of biomass could feasibly yield 66,676.25 units of pectin or 21,715.46 units of manganese peroxidase. Also, this study provides new information about methods and strategies for the expression of enzymes with industrial value. (C) 2015 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.es_ES
Doihttps://doi.org/10.1016/j.ejbt.2015.03.002es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4802
Idiomaenes_ES
EditorialUNIV CATOLICA DE VALPARAISOes_ES
RelaciónElectronic Journal of Biotechnologyes_ES
URL relacionadohttps://doi.org/10.1016/j.ejbt.2015.03.002es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteElectronic Journal of Biotechnology
Palabra claveCell walles_ES
Palabra claveChloroplast genetic transformationes_ES
Palabra claveHydrolytic enzymees_ES
Palabra claveNicotiana tabacumes_ES
TítuloStable expression and characterization of a fungal pectinase and bacterial peroxidase genes in tobacco chloroplastes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAlexander Espinoza-Sanchez, Edward
AutorHazel Alvarez-Hernandez, Marianela
AutorAriel Torres-Castillo, Jorge
AutorRascon-Cruz, Quintin
AutorGutierrez-Diez, Adriana
AutorZavala-Garcia, Francisco
AutorRamona Sinagawa-Garcia, Sugey
AutorAlexander Espinoza-Sanchez, Edwardes_ES
AutorHazel Alvarez-Hernandez, Marianelaes_ES
AutorAriel Torres-Castillo, Jorgees_ES
AutorRascon-Cruz, Quintines_ES
AutorGutierrez-Diez, Adrianaes_ES
AutorZavala-Garcia, Franciscoes_ES
AutorRamona Sinagawa-Garcia, Sugeyes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número3es_ES
Rango de páginas161-168es_ES
URL relacionadahttps://doi.org/10.1016/j.ejbt.2015.03.002
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen18es_ES

Files

Collections

Documentación Cientifica