Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758)

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:37Z
Fecha de publicación2011-01-01
ResumenBackground: The horn fly, Haematobia irritans (Linnaeus, 1758) (Diptera: Muscidae) is one of the most important ectoparasites of pastured cattle. Horn flies infestations reduce cattle weight gain and milk production. Additionally, horn flies are mechanical vectors of different pathogens that cause disease in cattle. The aim of this study was to conduct a functional genomics study in female horn flies using Expressed Sequence Tags (EST) analysis and RNA interference (RNAi). Results: A cDNA library was made from whole abdominal tissues collected from partially fed adult female horn flies. High quality horn fly ESTs (2,160) were sequenced and assembled into 992 unigenes (178 contigs and 814 singlets) representing molecular functions such as serine proteases, cell metabolism, mitochondrial function, transcription and translation, transport, chromatin structure, vitellogenesis, cytoskeleton, DNA replication, cell response to stress and infection, cell proliferation and cell-cell interactions, intracellular trafficking and secretion, and development. Functional analyses were conducted using RNAi for the first time in horn flies. Gene knockdown by RNAi resulted in higher horn fly mortality (protease inhibitor functional group), reduced oviposition (vitellogenin, ferritin and vATPase groups) or both (immune response and 5'-NUC groups) when compared to controls. Silencing of ubiquitination ESTs did not affect horn fly mortality and ovisposition while gene knockdown in the ferritin and vATPse functional groups reduced mortality when compared to controls. Conclusions: These results advanced the molecular characterization of this important ectoparasite and suggested candidate protective antigens for the development of vaccines for the control of horn fly infestations.es_ES
Doihttps://doi.org/10.1186/1471-2164-12-105es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5411
Idiomaenes_ES
EditorialBMCes_ES
RelaciónBMC Genomicses_ES
URL relacionadohttps://doi.org/10.1186/1471-2164-12-105es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteBMC Genomics
TítuloFunctional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758)es_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorTorres, Lorena
AutorAlmazan, Consuelo
AutorAyllon, Nieves
AutorGalindo, Ruth C.
AutorRosario-Cruz, Rodrigo
AutorQuiroz-Romero, Hector
Autorde la Fuente, Jose
AutorTorres, Lorenaes_ES
AutorAlmazan, Consueloes_ES
AutorAyllon, Nieveses_ES
AutorGalindo, Ruth C.es_ES
AutorRosario-Cruz, Rodrigoes_ES
AutorQuiroz-Romero, Hectores_ES
Autorde la Fuente, Josees_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
URL relacionadahttps://doi.org/10.1186/1471-2164-12-105
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen12es_ES

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