Amoebicidal activity of milk, apo-lactoferrin, slgA and lysozyme

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:34:29Z
Fecha de publicación2006-01-01
ResumenObjectives: To identify amoebicidal components in human milk and the effect of iron on the amoebicidal activity. Design: Investigation in axenic cultures of Entamoeba histolytica trophozoites. Methods: Amoebas were treated with 5%-20% of human, bovine and swine milk, with 10% of human milk fractions (i.e., casein, proteins except casein and fat) or with I mg/ml of human milk apo-lactoferrin, human secretory immunoglobulin type A (slgA) and chicken egg-white lysozyme (i.e., purified proteins). Milk proteins were detected using immunoblot. Confocal microscopy was used to define the interaction of milk proteins (100 μM each) and amoebas. Experiments were done at least three times in triplicate, and mean and standard deviations were calculated. Results: Human and bovine milk were amoebicidal showing a concentration-dependent effect. The amoebicidal effect was increased in the absence of iron. Milk protein fractions, with the exception of casein, were the components responsible for the amoebicidal activity found. Apo-lactoferrin, slgA and lysozyme were identified in the amoebicidal milk protein fraction. Apo-lactoferrin showed the major amoebicidal effect. These proteins, either alone or in combination, showed a killing effect on the trophozoites. They bound to the amoebic membrane causing cell rounding, lipid disruption and damage. Conclusions: Milk proteins such as apo-lactoferrin, slgA and lysozyme are able to kill Entamoeba histolytica trophozoites. This study confirms the importance of feeding breast milk to newborns.es_ES
Doihttps://doi.org/10.3121/cmr.4.2.106es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/6263
Idiomaenes_ES
RelaciónClinical Medicine and Researches_ES
URL relacionadohttps://doi.org/10.3121/cmr.4.2.106es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteClinical Medicine and Research
Palabra claveAmoebicidales_ES
Palabra claveApo-lactoferrines_ES
Palabra claveEntamoeba histolyticaes_ES
Palabra claveLysozymees_ES
Palabra claveMilkes_ES
Palabra claveSlgAes_ES
TítuloAmoebicidal activity of milk, apo-lactoferrin, slgA and lysozymees_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorLeón-Sicairos, Nidia
AutorLópez-Soto, Fernando
AutorReyes-López, Magda
AutorGodínez-Vargas, Delfino
AutorOrdaz-Pichardo, Cynthia
AutorDe La Garza, Mireya
AutorLeón-Sicairos, Nidiaes_ES
AutorLópez-Soto, Fernandoes_ES
AutorReyes-López, Magdaes_ES
AutorGodínez-Vargas, Delfinoes_ES
AutorOrdaz-Pichardo, Cynthiaes_ES
AutorDe La Garza, Mireyaes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número2es_ES
Rango de páginas106-113es_ES
URL relacionadahttps://doi.org/10.3121/cmr.4.2.106
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen4es_ES

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