mRNA levels of TLR4 and TLR5 are independent of H pylori
| Audiencia | Público en general | es_ES |
| Cobertura | México | es_ES |
| Fecha de ingreso | 2026-10-05T16:33:25Z | |
| Fecha de publicación | 2008-01-01 | |
| Resumen | AIM: To determine if the presence H pylori or its virulence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. METHODS: For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR. RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) and there were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological findings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition associated with the strains (P > 0.05 for both TLR4 and TLR5). CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both In vivo and in vitro, are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5. (c) 2008 The WJG Press. All rights reserved. | es_ES |
| Doi | https://doi.org/10.3748/wjg.14.5306 | es_ES |
| URI | https://riuat.uat.edu.mx/handle/123456789/5241 | |
| Idioma | en | es_ES |
| Editorial | BAISHIDENG PUBL GRP CO LTD | es_ES |
| Relación | World Journal of Gastroenterology | es_ES |
| URL relacionado | https://doi.org/10.3748/wjg.14.5306 | es_ES |
| Derechos | Acceso abierto (Metadatos de producción científica) | es_ES |
| Licencia | http://purl.org/coar/access_right/c_abf2 | es_ES |
| Fuente | World Journal of Gastroenterology | |
| Palabra clave | H pylori | es_ES |
| Palabra clave | Toll-like receptor 4 | es_ES |
| Palabra clave | Toll-like receptor 5 | es_ES |
| Palabra clave | AGS cells | es_ES |
| Palabra clave | mRNA | es_ES |
| Título | mRNA levels of TLR4 and TLR5 are independent of H pylori | es_ES |
| Tipo | Artículo | es_ES |
| Arbitrado | Ha sido Arbitrado | es_ES |
| Autor | Garza-Gonzalez, Elvira | |
| Autor | Bocanegra-Garcia, Virgilio | |
| Autor | Bosques-Padilla, Francisco Javier | |
| Autor | Flores-Gutierrez, Juan Pablo | |
| Autor | Moreno, Francisco | |
| Autor | Perez-Perez, Guillermo Ignacio | |
| Autor | Garza-Gonzalez, Elvira | es_ES |
| Autor | Bocanegra-Garcia, Virgilio | es_ES |
| Autor | Bosques-Padilla, Francisco Javier | es_ES |
| Autor | Flores-Gutierrez, Juan Pablo | es_ES |
| Autor | Moreno, Francisco | es_ES |
| Autor | Perez-Perez, Guillermo Ignacio | es_ES |
| Institución | Universidad Autónoma de Tamaulipas | |
| Institución | Universidad Autónoma de Tamaulipas | es_ES |
| Número | 34 | es_ES |
| Rango de páginas | 5306-5310 | es_ES |
| URL relacionada | https://doi.org/10.3748/wjg.14.5306 | |
| Tipo de artículo | Indexado | |
| Tipo de artículo | Indexado | es_ES |
| Volumen | 14 | es_ES |