Cytologic and biochemical changes associated with inoculation of amniotic fluid and meconium into lungs of neonatal rats

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:48Z
Fecha de publicación2001-01-01
ResumenObjective-To evaluate the effect of homologous amniotic fluid and meconium inoculated intratracheally into the lungs of neonatal rats. Animals-153 male 7-day-old Fischer-344 rats. Procedure-Amniotic fluid was obtained by cesarean section from the uterus of pregnant rats and meconium was collected at the time of birth from the gastrointestinal tract of neonatal rats. Neonatal rats were randomly allocated into 5 treatment groups. Two groups received 0.05 ml of saline (0.9\% NaCl) solution; the third and fourth groups received 0.05 ml of 50\% or 100\% amniotic fluid, respectively; the fifth group was inoculated with 0.05 ml of a 20\% suspension of meconium. Six or 7 rat pups/group were euthanatized by exsanguination under halothane anesthesia at postinoculation days 1, 3, 7, and 14. The magnitude of injury and inflammatory response was determined by biochemical and cytologic analyses of bronchoalveolar lavage fluid. Results-Inoculation with saline solution and amniotic fluid did not induce pulmonary injury or inflammatory response. Inoculation with meconium induced significant (P < 0.01) injury and inflammatory response, characterized by the release of cytosolic enzymes and recruitment of neutrophils in the lung. Conclusions-Saline solution is an innocuous vehicle that can be safely used in intratracheal inoculations in neonatal rats. Homologous amniotic fluid, despite containing keratin and epidermal cells, does not cause acute injury or inflammation in the lung. In contrast, meconium acts as a toxic substance injuring respiratory cells and causing a vigorous but transient leukocytic inflammatory reaction in the lungs.es_ES
Doihttps://doi.org/10.2460/ajvr.2001.62.1636es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4639
Idiomaenes_ES
EditorialAMER VETERINARY MEDICAL ASSOCes_ES
RelaciónAmerican Journal of Veterinary Researches_ES
URL relacionadohttps://doi.org/10.2460/ajvr.2001.62.1636es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteAmerican Journal of Veterinary Research
TítuloCytologic and biochemical changes associated with inoculation of amniotic fluid and meconium into lungs of neonatal ratses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorMartínez-Burnes, J
AutorLópez, A
AutorHorney, B
AutorMacKenzie, A
AutorBrimacombe, M
AutorMartínez-Burnes, Jes_ES
AutorLópez, Aes_ES
AutorHorney, Bes_ES
AutorMacKenzie, Aes_ES
AutorBrimacombe, Mes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número10es_ES
Rango de páginas1636-1641es_ES
URL relacionadahttps://doi.org/10.2460/ajvr.2001.62.1636
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen62es_ES

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