Electrophoretic analysis of whole saliva and prevalence of dental caries. A study in Mexican dental students

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:43Z
Fecha de publicación2002-01-01
ResumenBackground. Variability in salivary proteins and their posttranslational modifications may play an important role in determining their protective features against dental caries. Knowledge of molecular content of saliva in different populations is important for a better understanding of protective properties of this biological fluid. Aims of this study were to analyze electrophoretic pattern and protein composition in resting human whole saliva (HWS) of a Mexican population and to correlate these data with decayed, missing, and filled teeth (DMFT) index in these subjects. Methods. Resting human whole saliva samples were collected from 120 healthy Mexican dental students. Salivary flow rate, protein concentration, and electrophoretic profile analyzed qualitatively by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were correlated with DMIFT index. Gels were successively triple-stained with Coomassie brilliant blue R250, periodic acid Schiff (PAS), silver stain, and salivary molecules were scored as absent (-), present (+/-), and high intensity and size (+). Results. These showed no substantial differences in number of bands between males and females; however, a slight correlation between total protein concentration and sex was found (p less than or equal to0.05). With regard to salivary proteins and DMFT index, subjects with higher DMFT indices presented significant reduction or absence of high-molecular-weight mucin glycoprotein-1 (MG1), low-molecular-weight mucin glycoprotein-2 (MG2), and acidic proline-rich protein-1 (PRP-1), differing from subjects with lower DMFT indices (p less than or equal to0.001). Conclusions. This study concludes that genetic phenotypic polymorphism is present in the population studied and has correlations with oral health. We found specific characteristics and individual variability in number, intensity, and apparent molecular weight of band features in this Mexican population. These studies provide the initial step for creating an HWS database in this population. (C) 2002 IMSS. Published by Elsevier Science Inc.es_ES
Doihttps://doi.org/10.1016/s0188-4409(02)00395-8es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4546
Idiomaenes_ES
EditorialELSEVIER SCIENCE INCes_ES
RelaciónArchives of Medical Researches_ES
URL relacionadohttps://doi.org/10.1016/s0188-4409(02)00395-8es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteArchives of Medical Research
Palabra clavesalivaes_ES
Palabra claveelectrophoresises_ES
Palabra claveproteinses_ES
Palabra clavedental carieses_ES
TítuloElectrophoretic analysis of whole saliva and prevalence of dental caries. A study in Mexican dental studentses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorBanderas-Tarabay, JA
AutorZacarías-D'Oleire, IG
AutorGarduño-Estrada, R
AutorAceves-Luna, E
AutorGonzález-Begné, M
AutorBanderas-Tarabay, JAes_ES
AutorZacarías-D'Oleire, IGes_ES
AutorGarduño-Estrada, Res_ES
AutorAceves-Luna, Ees_ES
AutorGonzález-Begné, Mes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número5es_ES
Rango de páginas499-505es_ES
URL relacionadahttps://doi.org/10.1016/s0188-4409(02)00395-8
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen33es_ES

Files

Collections

Documentación Cientifica