In vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperda

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:02Z
Fecha de publicación2019-01-01
ResumenThe control of Spodoptera frugiperda is based on synthetic insecticides, so some alternatives are the use of entomopathogenic fungi (EF) and neem extract. The objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on S. frugiperda larvae. Six EF were identified by DNA sequencing of ITS regions from three EF (Fusarium solani, Metarrhizium robertsii, Nigrospora spherica and Penicillium citrinum). They were evaluated in concentrations of 1 x 10 8 spores/ mL. In addition, a second bioassay was carried out evaluating only F. solani, M. robertsii and N. sphaerica and the addition of vegetable oil. On the other hand, extraction of secondary metabolites from neem seed (Azadirachta indica) was carried out by performing, mass (g) and solvent volume (mL ethanol and water) combinations, which were subjected to microwaves and ultrasound. Subsequently, these extracts were evaluated in concentrations of 3\%, 4\% and 5\%. A survival analysis was performed for each of the bioassays. With respect to the results of the first bioassay, F. solani obtained a probability of survival of 0.476 on the seventh day, while in the second bioassay, M. robertsii obtained 0.488 survival probability. This suggests that the expected percentage of larvae that stay alive on the sixth day is 48.8\%. However, in the evaluation of the neem extract the combination 1:12/70\% to 4\% caused 84\% mortality of larvae. The use of native HE and neem extracts has potential for the control of S. frugiperda.es_ES
Doihttps://doi.org/10.32604/phyton.2019.04611es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4878
Idiomaenes_ES
EditorialTECH SCIENCE PRESSes_ES
RelaciónPhytones_ES
URL relacionadohttps://doi.org/10.32604/phyton.2019.04611es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuentePhyton
Palabra claveBioinsecticidees_ES
Palabra claveBiological controles_ES
Palabra claveFall armywormes_ES
Palabra claveExtractses_ES
Palabra claveSeedes_ES
TítuloIn vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperdaes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorHernandez-Trejo, A.
AutorEstrada-Drouaillet, B.
AutorLopez-Santillan, J. A.
AutorRios-Velasco, C.
AutorVarela-Fuentes, S. E.
AutorRodriguez-Herrera, R.
AutorOsorio-Hernandez, E.
AutorHernandez-Trejo, A.es_ES
AutorEstrada-Drouaillet, B.es_ES
AutorLopez-Santillan, J. A.es_ES
AutorRios-Velasco, C.es_ES
AutorVarela-Fuentes, S. E.es_ES
AutorRodriguez-Herrera, R.es_ES
AutorOsorio-Hernandez, E.es_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número1es_ES
Rango de páginas47-54es_ES
URL relacionadahttps://doi.org/10.32604/phyton.2019.04611
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen88es_ES

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