Production of Microbial Transglutaminase on Media Made from Sugar Cane Molasses and Glycerol

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:32:51Z
Fecha de publicación2009-01-01
ResumenTransglutaminase is an enzyme that catalyses an acyl transfer reaction between gamma-carboxamide groups of glutaminyl residues and lysine residues in proteins. Due to this property, this enzyme is used for enhancing textural properties of protein-rich food. The transglutaminase used as food additive is obtained by microorganisms, mainly by Streptoverticillium ladakanum. On the other hand, sugar cane molasses is a viscous liquid rich in noncrystallized carbohydrates (saccharose, glucose and fructose). In this work, the feasibility of using sugar cane molasses as a carbon source for the production of microbial transglutaminase by Streptoverticillium ladakanum NRRL 3191 has been studied. Carbon sources including sugar cane molasses (60 g of total sugars per L), glycerol (60 g/L) and their mixture in a ratio of 1:1 (30 g/L of each) were evaluated. Time course of microbial growth, transglutaminase activity and carbon source consumption were determined every 24 h during 120 h of fermentations at three agitation speeds (200, 300 or 400 rpm). The results showed that with the increase in agitation speed, the biomass concentration increased up to 8.39 g/L in the medium containing sugar cane molasses alone or the mixture of molasses and glycerol. The highest transglutaminase activity was obtained at 400 rpm in the medium containing a mixture of molasses and glycerol, reaching 0.460 U/mL, while in the medium containing sugar cane molasses alone, the activity was 0.240 U/mL, and using glycerol alone it was 0.250 U/mL. These results show that sugar cane molasses is a suitable medium for transglutaminase production when it is combined with glycerol.es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/4690
Idiomaenes_ES
EditorialFACULTY FOOD TECHNOLOGY BIOTECHNOLOGYes_ES
RelaciónFood Technology and Biotechnologyes_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteFood Technology and Biotechnology
Palabra clavetransglutaminasees_ES
Palabra claveglyceroles_ES
Palabra claveglucosees_ES
Palabra clavesugar cane molasseses_ES
TítuloProduction of Microbial Transglutaminase on Media Made from Sugar Cane Molasses and Glyceroles_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorPortilla-Rivera, Oscar M.
AutorTellez-Luis, Simon J.
Autorde Leon, Jose A. Ramirez
AutorVazquez, Manuel
AutorPortilla-Rivera, Oscar M.es_ES
AutorTellez-Luis, Simon J.es_ES
Autorde Leon, Jose A. Ramirezes_ES
AutorVazquez, Manueles_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número1es_ES
Rango de páginas19-26es_ES
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen47es_ES

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