Assessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassays

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:31:13Z
Fecha de publicación2006-01-01
ResumenHyperprolactinemia leads to several diagnoses, however, the immunoassay used for the prolactin determination and the presence of macroprolactin, could induce a misdiagnosis. The aim of this study was to evaluate the usefulness of separation and detection of macroprolactin by precipitation with polyethylene glycol, using two commercial immunoassays, Axsym Abbott and Advia Centaur Bayer in patients with established diagnosis of hyperprolactinemia. One hundred and seventeen samples were tested. Samples were obtained from female patients aged between 22 to 59 years old. We determined total prolactin (PRL), macroprolactin and free prolactin. In 30 of these samples hyperprolactinemia was detected. PRL recovering percentages of supernatant fraction were from 6.5% to 78.5% and from 26.2% to 75.4% when we compared Advia to Axsym systems initial values, respectively. Based on the PRL recovering percentage, 7 samples with significant macro-PRL presence were observed. The macroprolactin prevalence in these 30 samples, after PEG precipitation, was of 23% and 10% in the Axsym and Advia system respectively. Based upon these findings, we establish that references values need to be determined for both instruments, having applied the polyethylene glycol precipitation for the prolactine determination.es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2905
Idiomaenes_ES
RelaciónBioquimiaes_ES
URL relacionadohttps://www.redalyc.org/pdf/576/57631404.pdfes_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteBioquimia
Palabra clavePolyethylene glycoles_ES
Palabra claveProlactines_ES
Palabra claveImmunoassayes_ES
Palabra claveChromatographyes_ES
Palabra claveInternal medicinees_ES
Palabra clavePEG ratioes_ES
Palabra claveEndocrinologyes_ES
Palabra claveMedicinees_ES
Palabra claveChemistryes_ES
Palabra claveImmunologyes_ES
Palabra claveBiochemistryes_ES
Palabra claveHormonees_ES
ClasificaciónPituitary Gland Disorders and Treatmentses_ES
TítuloAssessment of macroprolactin after polyethylene glycol precipitation in two commercial immunoassayses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorRivas-Espinosa, Jesús
AutorTrigos-Landa, Ángel
AutorBocanegra‐García, Virgilio
AutorBocanegra-Alonso, Anabel
AutorRivera, Gildardo
AutorRivas-Espinosa, Jesúses_ES
AutorTrigos-Landa, Ángeles_ES
AutorBocanegra‐García, Virgilioes_ES
AutorBocanegra-Alonso, Anabeles_ES
AutorRivera, Gildardoes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número4es_ES
Rango de páginas140-145es_ES
URL relacionadahttps://www.redalyc.org/pdf/576/57631404.pdf
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen31es_ES

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