Safety and efficacy of intravesical instillation of resiniferatoxin in healthy cats: A preliminary study

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:38Z
Fecha de publicación2023-01-01
ResumenObjectivesTo evaluate the safety of intravesical application of resiniferatoxin (RTX) in healthy cats and its effects on calcitonin gene-related peptide (CGRP) and substance P (SP) produced by C-fibers. MethodsSeven adult female cats received either 25 mL of saline (control; n = 1), or intravesical RTX at 5, 25, or 50 mu g in 25 mL of saline to a final concentration of 0.2 mu g/mL (318 nM), 1 mu g/mL (1,591 nM), and 2 mu g/mL (3,181 nM) (n = 2 per group). The treatment was instilled into the urinary bladder for 20 min. Plasma concentrations of RTX were measured at 0, 0.5, 1, and 4 h. Physical exam, complete blood count, and serum biochemical analysis were performed on day 0, 7, and 14. After 14 days, the sacral dorsal root ganglia (DRG) and the urinary bladder were harvested for histological and immunofluorescence analysis. ResultsIntravesical RTX was well tolerated and plasma concentrations were below the quantifiable limits except for one cat receiving 1 mu g/mL. Mild to moderate histopathological changes, including epithelial changes, edema, and blood vessel proliferation, were observed at lower doses (0.2 and 1 mu g/mL), and were more severe at the higher dose (2 mu g/mL). C-fiber ablation was observed in the urinary bladder tissue at all doses, as shown by an apparent reduction of both CGRP and SP immunoreactive axons. ConclusionA dose of 25 mu g (1 mu g/mL) of RTX instilled in the urinary bladder of healthy cats appeared to decrease the density of SP and CGRP nerve axons innervating bladder and induced moderate changes in the bladder tissue.es_ES
Doihttps://doi.org/10.3389/fvets.2022.922305es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5444
Idiomaenes_ES
EditorialFRONTIERS MEDIA SAes_ES
RelaciónFrontiers in Veterinary Sciencees_ES
URL relacionadohttps://doi.org/10.3389/fvets.2022.922305es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteFrontiers in Veterinary Science
Palabra claveC-fiberses_ES
Palabra clavefelinees_ES
Palabra claveinterstitial cystitises_ES
Palabra clavepaines_ES
Palabra clavesubstance Pes_ES
Palabra claveurinary bladderes_ES
Palabra clavecalcitonin gene-related peptide (CGRP)es_ES
TítuloSafety and efficacy of intravesical instillation of resiniferatoxin in healthy cats: A preliminary studyes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorBarletta, Michele
AutorGordon, Julie
AutorEscobar, Andre
AutorMitchell, Krista
AutorTrenholme, H. Nicole
AutorGrimes, Janet A. A.
AutorJimenez-Andrade, Juan M. M.
AutorNahama, Alexis
AutorCisternas, Alvaro
AutorBarletta, Michelees_ES
AutorGordon, Juliees_ES
AutorEscobar, Andrees_ES
AutorMitchell, Kristaes_ES
AutorTrenholme, H. Nicolees_ES
AutorGrimes, Janet A. A.es_ES
AutorJimenez-Andrade, Juan M. M.es_ES
AutorNahama, Alexises_ES
AutorCisternas, Alvaroes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
URL relacionadahttps://doi.org/10.3389/fvets.2022.922305
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen9es_ES

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