An in vitro study of ApxI from Actinobacillus pleuropneumoniae serotype 10 and induction of NLRP3 inflammasome-dependent cell death

Simple item page
AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:18Z
Fecha de publicación2021-01-01
ResumenBackground Actinobacillus pleuropneumoniae (AP) is the causative agent of porcine pleuropneumonia. Apx exotoxins are the most important virulence factors associated with the induction of lesions. ApxI is highly cytotoxic on a wide range of cells. Besides the induction of necrosis and apoptosis of ApxI on porcine alveolar macrophages (PAMs), its role in pyroptosis, a caspase-1-dependent form of cell death, has not been reported. The aim of this study was to analyse if NLRP3 inflammasome participates in cell death induced by ApxI. Methods PAMs, the porcine alveolar macrophage cell line 3D4/21 and a porcine aortic endothelial cell line were used in this study. We used Z-VAD-FMK and Ac-YVAD-cmk to inhibit caspase-1. Glyburide and MCC950 were used to inhibit the NLRP3 inflammasome. A lactate dehydrogenase release assay was used to measure the percentage of cell death. Caspase-1 expression was analysed by immunofluorescence. End-point RT-PCR was used to analyse the expression of NLRP3 mRNA. Results Rapid cell death in PAMs, 3D4/21 cells and the endothelial cell line were induced by ApxI. This cell death decreased by using caspase-1 and NLRP3 inflammasome inhibitors and by blocking the K+ efflux. Expression of NLRP3 mRNA was induced by ApxI in alveolar macrophages while it was constitutive in the endothelial cell line. Detection of caspase-1 in alveolar macrophages was higher after ApxI treatment and it was blocked by MCC950 or heat inactivation. Conclusions To the best of the authors' knowledge, we have described for the first time in vitro induction of ApxI associated pyroptosis in alveolar macrophages and endothelial cells, a rapid cell death that depends on the activation of caspase-1 via the NLRP3 inflammasome.es_ES
Doihttps://doi.org/10.1002/vro2.20es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5123
Idiomaenes_ES
EditorialJOHN WILEY \& SONS LTDes_ES
RelaciónVeterinary Record Openes_ES
URL relacionadohttps://doi.org/10.1002/vro2.20es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteVeterinary Record Open
TítuloAn in vitro study of ApxI from Actinobacillus pleuropneumoniae serotype 10 and induction of NLRP3 inflammasome-dependent cell deathes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorHernandez-Cuellar, Eduardo
AutorLilian Guerrero-Barrera, Alma
AutorJavier Avelar-Gonzalez, Francisco
AutorManuel Diaz, Juan
AutorChavez-Reyes, Jesus
AutorSalazar de Santiago, Alfredo
AutorHernandez-Cuellar, Eduardoes_ES
AutorLilian Guerrero-Barrera, Almaes_ES
AutorJavier Avelar-Gonzalez, Franciscoes_ES
AutorManuel Diaz, Juanes_ES
AutorChavez-Reyes, Jesuses_ES
AutorSalazar de Santiago, Alfredoes_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número1es_ES
URL relacionadahttps://doi.org/10.1002/vro2.20
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen8es_ES

Files

Collections

Documentación Cientifica