Identification and characterization of Rhipicephalus (Boophilus) microplus candidate protective antigens for the control of cattle tick infestations

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-10-05T16:33:40Z
Fecha de publicación2010-01-01
ResumenThe cattle ticks, Rhipicephalus (Boophilus) spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant Rhipicephalus microplus Bm86 antigen has been shown to protect cattle against tick infestations. However, variable efficacy of Bm86-based vaccines against geographic tick strains has encouraged the research for additional tick-protective antigens. Herein, we describe the analysis of R. microplus glutathione-S transferase, ubiquitin (UBQ), selenoprotein W, elongation factor-1 alpha, and subolesin (SUB) complementary DNAs (cDNAs) by RNA interference (RNAi) in R. microplus and Rhipicephalus annulatus. Candidate protective antigens were selected for vaccination experiments based on the effect of gene knockdown on tick mortality, feeding, and fertility. Two cDNA clones encoding for UBQ and SUB were used for cattle vaccination and infestation with R. microplus and R. annulatus. Control groups were immunized with recombinant Bm86 or adjuvant/saline. The highest vaccine efficacy for the control of tick infestations was obtained for Bm86. Although with low immunogenic response, the results with the SUB vaccine encourage further investigations on the use of recombinant subolesin alone or in combination with other antigens for the control of cattle tick infestations. The UBQ peptide showed low immunogenicity, and the results of the vaccination trial were inconclusive to assess the protective efficacy of this antigen. These experiments showed that RNAi could be used for the selection of candidate tick-protective antigens. However, vaccination trials are necessary to evaluate the effect of recombinant antigens in the control of tick infestations, a process that requires efficient recombinant protein production and formulation systems.es_ES
Doihttps://doi.org/10.1007/s00436-009-1689-1es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/5475
Idiomaenes_ES
EditorialSPRINGERes_ES
RelaciónParasitology Researches_ES
URL relacionadohttps://doi.org/10.1007/s00436-009-1689-1es_ES
DerechosAcceso abierto (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_abf2es_ES
FuenteParasitology Research
TítuloIdentification and characterization of Rhipicephalus (Boophilus) microplus candidate protective antigens for the control of cattle tick infestationses_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorAlmazan, Consuelo
AutorLagunes, Rodolfo
AutorVillar, Margarita
AutorCanales, Mario
AutorRosario-Cruz, Rodrigo
AutorJongejan, Frans
Autorde la Fuente, Jose
AutorAlmazan, Consueloes_ES
AutorLagunes, Rodolfoes_ES
AutorVillar, Margaritaes_ES
AutorCanales, Marioes_ES
AutorRosario-Cruz, Rodrigoes_ES
AutorJongejan, Franses_ES
Autorde la Fuente, Josees_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número2es_ES
Rango de páginas471-479es_ES
URL relacionadahttps://doi.org/10.1007/s00436-009-1689-1
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen106es_ES

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