Effect of oxalic acid on postharvest life of tomato modified with the TomLoxB gene in antisense

Abstract

Oxalic acid is an organic compound found in green leafy vegetables, which has proven to be effective in delaying ripening by inhibiting ethylene synthesis in fruits such as banana, mango, peach, tomato, plum, and others. In this study, the response to oxalic acid application on postharvest physiology was evaluated in transgenic tomatoes ( Solanum lycopersicum) varietyTA234 with the TomLoxB gene insertion in antisense, at two concentrations of oxalic acid: 3 and 10 mM, during 30 d of storage at 25 +/- 1 degrees C and a relative humidity of 65- 70 \%. The fruits were harvested at break stage and immersed for 10 minutes in the oxalic acid solution, which was maintained at 25 degrees C. Upon treatment, less weight loss, better retention of lightness, delay in the decrease of firmness and hue angle, decrease in lipoxygenase activity, low electrolyte leakage and increase in total phenolics content were observed. The most effective oxalic acid concentration was 3 mM, that extended postharvest life by up to 30 d and reduced deterioration of the genetically modified (GM) tomatoes. In turn, the untreated GM tomatoes showed an acceptable appearance up to day 24 of storage, while the wild type fruits were kept satisfactorily for 15 d.