Optimization of peroxide-alkaline pretreatment and enzymatic hydrolysis of barley straw (Hordeum vulgare L.) to produce fermentable sugars using a Box–Behnken design

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AudienciaPúblico en generales_ES
CoberturaMéxicoes_ES
Fecha de ingreso2026-09-15T00:30:36Z
Fecha de publicación2022-01-01
ResumenIn recent years, interest has increased in the use of agro-industrial wastes for use as raw materials in the production of second-generation biofuels and other products of industrial interest. A pretreatment is necessary, followed by an enzymatic hydrolysis to obtain high yields of fermentable sugars. In the present study, the best conditions were established both for peroxide-alkaline pretreatment and for enzymatic hydrolysis of the Mexican barley straw (Hordeum vulgareL.) variety Dona Josefa in order to maximize lignin removal and improve enzyme conversion. Response surface methodology based on a Box-Behnken experimental design was used to optimize pretreatment conditions (concentration of hydrogen peroxide {[}H2O2], reaction time, and liquid-solid ratio (LSR)), and enzymatic hydrolysis (cellulase and xylanase concentrations and reaction time). Samples obtained from residual bagasse at each process stage were analyzed by means of scanning electron microscopy (SEM) and infrared spectrometry (FTIR) to evaluate the changes in the structure of the material after treatments. In pretreatment optimization, it was possible to obtain 60\% delignification at a 6\% peroxide concentration and a 12:1 LSR in 24 h. In saccharification, a maximum 82\% conversion of cellulose to glucose using 6\% cellulase (Cellic (R) CTec III), and 4\% xylanase (Cellic (R) HTec III) in 20 h of hydrolysis, was used in obtaining a maximum sugar concentration of 70 g/L. Peroxide-alkaline pretreatment together with state-of-the-art enzyme saccharification makes barley straw an excellent alternative for fermentable sugar production that can be used for various biotech industries.es_ES
Doihttps://doi.org/10.1007/s13399-020-00853-4es_ES
URIhttps://riuat.uat.edu.mx/handle/123456789/2550
Idiomaenes_ES
EditorialSpringer Naturees_ES
RelaciónBiomass Conversion and Biorefineryes_ES
URL relacionadohttps://doi.org/10.1007/s13399-020-00853-4es_ES
DerechosAcceso restringido / Suscripción (Metadatos de producción científica)es_ES
Licenciahttp://purl.org/coar/access_right/c_16eces_ES
FuenteBiomass Conversion and Biorefinery
Palabra claveCellulasees_ES
Palabra claveChemistryes_ES
Palabra claveBagassees_ES
Palabra claveEnzymatic hydrolysises_ES
Palabra claveXylanasees_ES
Palabra claveHydrolysises_ES
Palabra claveHordeum vulgarees_ES
Palabra claveXylosees_ES
Palabra claveReducing sugares_ES
Palabra claveHydrogen peroxidees_ES
Palabra claveCellulosees_ES
Palabra claveLignines_ES
ClasificaciónBiofuel production and bioconversiones_ES
TítuloOptimization of peroxide-alkaline pretreatment and enzymatic hydrolysis of barley straw (Hordeum vulgare L.) to produce fermentable sugars using a Box–Behnken designes_ES
TipoArtículoes_ES
ArbitradoHa sido Arbitradoes_ES
AutorIbarra-Díaz, N.
AutorCastañón‐Rodríguez, Juan Francisco
AutorIbarra-Díaz, N.es_ES
AutorRodríguez, Javier Gómez
AutorCastañón‐Rodríguez, Juan Franciscoes_ES
AutorRodríguez, Javier Gómezes_ES
AutorAguilar‐Uscanga, María Guadalupe
AutorAguilar‐Uscanga, María Guadalupees_ES
InstituciónUniversidad Autónoma de Tamaulipas
InstituciónUniversidad Autónoma de Tamaulipases_ES
Número7es_ES
Rango de páginas2389-2398es_ES
URL relacionadahttps://doi.org/10.1007/s13399-020-00853-4
Tipo de artículoIndexado
Tipo de artículoIndexadoes_ES
Volumen12es_ES

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